儿童白血病患者血浆循环DNA检测及临床意义  

Auantitative analysis of circulating DNA in plasma from pediatric acute leukemia patients

作　　者：孙开胜[1] 邹爱军[2] 李子博[1] 左雅婵[1] 王留杰[1] 吴安山[1] 

机构地区：[1]长沙医学院检验系,湖南长沙415200 [2]湖南省儿童医院血液科,湖南长沙415200

出　　处：《长沙医学院学报》2008年第2期29-31,16,共4页Journal of Changsha Medical University

基　　金：湖南省教育厅科学研究项目(项目编号:07c153)

摘　　要：目的:通过对儿童白血病患者血浆循环DNA定量检测,分析血浆循环DNA含量用于儿童白血病诊断的价值。方法:用血液微量DNA抽提试剂盒提取血浆循环DNA,以SYBR GreenⅠ荧光染色法分别检测46例儿童急性白血病患者、20例健康对照标本血浆循环DNA含量;利用受试者工作特征(ROC)曲线评价血浆循环DNA对儿童白血病的诊断价值。结果:儿童白血病患者血浆循环DNA含量为(30.04±15.13)ng/mL,高于对照组(12.22±7.10)ng/mL,二者差异有统计学意义(P<0.05);循环DNA含量16.3l ng/mL为诊断儿童白血病最佳临界值,敏感性和特异度分别为86.96%和75.00%, ROC曲线下面积(AUC)为0.879。结论:血浆循环DNA定量检测有可能成为一种新的用于儿童白血病诊断方法。Objective To evaluate the diagnostic value of DNAin plasma from pediatric acute leukemia patients.Methods The plasma DNA of 46 cases and 20 controls were extracted and purified by QIAamp DNA Blood Mini Kit.The Quantitative measurement of plasma DNA was performed by quantitative fluorescence based assay using SYBR greenⅠand standard ultraviolet transilluminator system.Results The mean value of plasma DNA concentration in pediatric acute leukemia patients was(30.04±15.13) ng/mL,which was significantly higher(P<0.05)than that of the healthy controls,(12.22±7.10)ng/mL. The optimum cut-off value of plasma DNA concentration was 16.31 ng/mL The area under receiver operating characteristic curve(AUC)of plasma DNA was 0.879.and the sensitivity and specificity were 86.96%and 75.00%respectively.Conclusion The plasma DNA in pediatric acute leukemia patients was higher than that of the healthy controls.Quantification of plasma DNA may develop into a new assay for the diagnosis of pediatric acute leukemia.

关 键 词：儿童白血病 循环DNA ROC曲线 

分 类 号：R733.7[医药卫生—肿瘤]