Transfection of bone marrow mesenchymal stem cells using green fluorescence protein labeled hVEGF165 recombinant plasmid mediated by liposome  

作　　者：Tao Wang Tian-An Liao Shao-Bo Zhong 

机构地区：[1]Dental Medical Center,People's Hospital of Hainan Province

出　　处：《Asian Pacific Journal of Tropical Medicine》2013年第9期739-742,共4页亚太热带医药杂志（英文版）

基　　金：supported by grants from the National Natural Science Foundation of Hainan Province(30635);Foundation of Health Department of Hainan Province(2008-40)

摘　　要：Objective:To study the role of bone marrow mesenchymal stem cells(BMSCs)in construction of vascularized engineered tissue.Methods:hVEGF165 was amplified via RT-PCR before recombinant with pShuttle-green fluorescence protein;green fluorescent protein(GFP)-CMV.Then the recombinant shuttle plasmid was transfected into BMSCs with Lipofectamine^(TM)2000 for packaging and amplifying.hVTGF165 mRNA expression in BMSCs cells was tested.Results:The sequence of hVEGFI65 in pShutlle-GFP-hVFGF165 plasmid was confirimed by double-enzyme cleavage method and sequencing.hVECF165 was highly expressed in BMSCs.Conclusions:The GFP/hVECF165 recombinant plasmid vector was constructed successfully and expressed effectively in host cells,which may be helpful for discussing the possibility of the application of VEGF165-BMSCs in tissue engineering and ischemic disease cure.Objective:To study the role of bone marrow mesenchymal stem cells（BMSCs）in construction of vascularized engineered tissue.Methods:hVEGF165 was amplified via RT-PCR before recombinant with pShuttle-green fluorescence protein;green fluorescent protein（GFP）-CMV.Then the recombinant shuttle plasmid was transfected into BMSCs with LipofectamineTM2000 for packaging and amplifying.hVTGF165 mRNA expression in BMSCs cells was tested.Results:The sequence of hVEGFI65 in pShutlle-GFP-hVFGF165 plasmid was confirimed by double-enzyme cleavage method and sequencing.hVECF165 was highly expressed in BMSCs.Conclusions:The GFP/hVECF165 recombinant plasmid vector was constructed successfully and expressed effectively in host cells,which may be helpful for discussing the possibility of the application of VEGF165-BMSCs in tissue engineering and ischemic disease cure.

关 键 词：Vascular endothelial growth factor Green fluorescent protein Bone MARROW MESENCHYMAL stem cells PLASMID 

分 类 号：R341[医药卫生—基础医学]