Anthelmintic activity of Securidaca longepedunculata(Family:Polygalaceae)root extract in mice,in vitro and in vivo  

作　　者：Adiele RC Fakae BB Isuzu IU 

机构地区：[1]Department of Anatomy and Cell Biology.Division of Biomedical Sciences,College of Medicine,University of Saskatchewan,Saskatoon.SK.S7N5E [2]Cameco MS Neuroscience Research Center,Saskatoon City Hospital,Saskatoon,SK,S7K OM7 [3]Department of Veterinary Parasitology and Entomology,Faculty of Veterinary Medicine,University of Nigeria,Nsukka [4]Vice Chancellor's Office,River State university of Science and Technology,Port Harcourt [5]Department of Veterinary Physiology and Pharmacology,Faculty of Veterinary Medicine,University of Nigeria,Nsukka [6]Deputy Vice Chancellor's Office.University of Nigeria,Nsukka

出　　处：《Asian Pacific Journal of Tropical Medicine》2013年第11期841-846,共6页亚太热带医药杂志（英文版）

摘　　要：Objective:To elucidate the pharmacological bases of oral administration of Securidaca longepedunculata(S.longepedunculata) root extract as an anthelmintic in folkloric medicine. Methods:Albino mice were infected with infective third(L3) larval stage of Heligmosomoides polygyrus(H.polygyrus) by esophageal intubation.Following establishment of the adult worms in the intestine,the mice were treated with 0-2 000 mg/kg body weight(bw) of methanolic root extract of S.longepedunculata and 100 mg/kg bw of pyrantel embonate,the reference drug in vivo. Bioactivity and larvicidal effects of the extract were tested by exposing brine shrimps(Artemia salina) to 0.00-1.00 mg/mL and the L3 stage of Heligmosomoides contortus(H.contortus) and H.polygyrus to 0.00-2.50 mg/mL of the extract in vitro.Results:The percentage yield of the extract was 7.13%w/w dry matter.The brine shrimps toxicity bioassay resulted in an LC_(50) of 74.18 μ g/mL.The extract had a significant,dose-dependent larvicidal effect on the L3 stage of H.contortus and H.polygyrus with the terminal effect of 75%and 70%at the highest exposure concentrations,respectively.The extract however,did not affect the number of worm eggs per gram(epg) of fecal materials(P<0.05) and total worm burden(twb) of adult H.polygyrus in infected mice.Treatment with pyrantel embonate significant reduced both the fecal egg count and twb to 0 compared to the untreated control(P<0.05).Conclusions:These results indicate that S.longepedunculata root extract contains potent bioactive compounds and has larvicidal effect on L3 stage of H.contortus and H.polygyrus,substantiating its use as anthelmintic in alternative medicine.Objective:To elucidate the pharmacological bases of oral administration of Securidaca longepedunculata（S.longepedunculata） root extract as an anthelmintic in folkloric medicine. Methods:Albino mice were infected with infective third（L3） larval stage of Heligmosomoides polygyrus（H.polygyrus） by esophageal intubation.Following establishment of the adult worms in the intestine,the mice were treated with 0-2 000 mg/kg body weight（bw） of methanolic root extract of S.longepedunculata and 100 mg/kg bw of pyrantel embonate,the reference drug in vivo. Bioactivity and larvicidal effects of the extract were tested by exposing brine shrimps（Artemia salina） to 0.00-1.00 mg/mL and the L3 stage of Heligmosomoides contortus（H.contortus） and H.polygyrus to 0.00-2.50 mg/mL of the extract in vitro.Results:The percentage yield of the extract was 7.13%w/w dry matter.The brine shrimps toxicity bioassay resulted in an LC50 of 74.18 μ g/mL.The extract had a significant,dose-dependent larvicidal effect on the L3 stage of H.contortus and H.polygyrus with the terminal effect of 75%and 70%at the highest exposure concentrations,respectively.The extract however,did not affect the number of worm eggs per gram（epg） of fecal materials（P&lt;0.05） and total worm burden（twb） of adult H.polygyrus in infected mice.Treatment with pyrantel embonate significant reduced both the fecal egg count and twb to 0 compared to the untreated control（P&lt;0.05）.Conclusions:These results indicate that S.longepedunculata root extract contains potent bioactive compounds and has larvicidal effect on L3 stage of H.contortus and H.polygyrus,substantiating its use as anthelmintic in alternative medicine.

关 键 词：ANTHELMINTIC Securidaca longepedunculata HAEMONCHUS contortus Heligmosomoides polygyrus Brine SHRIMPS LARVICIDAL FECAL egg count 

分 类 号：S853.7[农业科学—临床兽医学]