A comparative pharmacological and phytochemical analysis of in vivo & in vitro propagated Crotalaria species  

作　　者：Bellary Nagaraju Devendra Nakka Srinivas Kusuma Sandeep Solmon 

机构地区：[1]Department of Biotechnology,GITAM Institute of Science,GITAM University,Visakhapatnam-530 045,India [2]Department of Bioinformatics,GITAM Institute of Science,GITAM University,Visakhapatnam- 530 045,India

出　　处：《Asian Pacific Journal of Tropical Medicine》2012年第1期37-41,共5页亚太热带医药杂志（英文版）

摘　　要：Objective:To compare the efficacy for phytochemical.antibacterial and antioxidant activities of petroleum ether,chloroform,ethanol,and aqueous extracts of in vitro propagated plants and field grown plants of Crotalaria sps.,for against five human pathogens.Methods:The preliminary pbytochemistry,antimicrobial and antioxidant activities were evaluated using disc diffusion and DPPH radical scavenging methods.Results:The ethanolic extract of in vitro raised Crotalaria retusa（C.retusa） was effective on tested microorganisms and optimal ZOI values of 38 mm was obtained against Pseudomonas aeruginosa（P.aeruginosa）.The optimal concentration (IC₅₀ required for 50%inhibition of the DPPH radical scavenging was 57.6μg/mL obtained for ethanolic extract of in vitro propagated C.retusa.The in vitro propagated C.retusa has significant pharmacological activities while the Crotalaria prostrate（C.prostrate） and Crotalaria medicaginea（C.medicaginea） has low pharmacological activites.It was cleared that ethanolic extract of in vitro regenerated plants was most effective.Conclusions:These findings indicate compounds isolated from ethanolic extracts of Crotalaria sps..possesses pharmacological properties and potential to develop natural compounds based pharmaceutical products.The IC₅₀ values for ethanolic extracts of Crotalaria sps.was evaluated through the Linear regression analysis（R²≤1）.Objective:To compare the efficacy for phytochemical.antibacterial and antioxidant activities of petroleum ether,chloroform,ethanol,and aqueous extracts of in vitro propagated plants and field grown plants of Crotalaria sps.,for against five human pathogens.Methods:The preliminary pbytochemistry,antimicrobial and antioxidant activities were evaluated using disc diffusion and DPPH radical scavenging methods.Results:The ethanolic extract of in vitro raised Crotalaria retusa(C.retusa) was effective on tested microorganisms and optimal ZOI values of 38 mm was obtained against Pseudomonas aeruginosa(P.aeruginosa).The optimal concentration (IC_(50) required for 50%inhibition of the DPPH radical scavenging was 57.6μg/mL obtained for ethanolic extract of in vitro propagated C.retusa.The in vitro propagated C.retusa has significant pharmacological activities while the Crotalaria prostrate(C.prostrate) and Crotalaria medicaginea(C.medicaginea) has low pharmacological activites.It was cleared that ethanolic extract of in vitro regenerated plants was most effective.Conclusions:These findings indicate compounds isolated from ethanolic extracts of Crotalaria sps..possesses pharmacological properties and potential to develop natural compounds based pharmaceutical products.The IC_(50) values for ethanolic extracts of Crotalaria sps.was evaluated through the Linear regression analysis(R^2≤1).

关 键 词：COMPARATIVE STUDIES CROTALARIA SPECIES PHARMACOLOGICAL STUDIES PHYTOCHEMISTRY Antioxidant activity 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]