Analysis of var genes cloned from a Plasmodium falcivarum isolate in China  

作　　者：Ning Jiang Li Meng Hui-Jun Lu Wei Kang Shuai Peng Wei-Qing Pan Ji-Gang Yin Qi-Jun Chen 

机构地区：[1]Key Laboratory of Zoonosis,Ministry of Education,Institute of Zoonosis,Jilin University [2]Department of Pathogen Biology [3]Laboratory of Parasitology,Institute of Pathogen Biology,Chinese Academy of Medical Sciences

出　　处：《Asian Pacific Journal of Tropical Medicine》2012年第2期85-90,共6页亚太热带医药杂志（英文版）

基　　金：supports to Q.Chen from the National Basic Research Program of China(973 Program,No.2007CB513100);national science and technology project(2008ZX-10004-011);NSFC grant to J.Yin (30771886),N.Jiang(81171592,)and Q.Chen(81130033)

摘　　要：Objective:To analyse the rar gene repertoire and characterise the rhondroitin sulphate A (CSA)-binding activity of the Duffy-binding like(I)BI.) domains encoded by the var2csa gene of a Plasmodium falciparum(P.falciparum) isolate in Hainan Province,China.Methods:The sequences of var DBL1 regions were PCR-amplified,sequenced and the sequence characteristics was bioinformalically analysed.Recombinant proteins encoded by the var2csa genes were expressed and purified.The binding activities of the recombinant proteins to CSA receptor was detected by ELISA assays.Results:Fifty six unique DBI.a sequences were obtained,and the sequences represented similar diversity to the var genes of the genome parasite 3D7.There are two var2csa genes in the P.falciparum isolated from Hainan Province.Unlike in other falciparum parasites such as HB3,the two var2csa genes are more diverged.The receptor-binding capacity of DBL-5εand DBI.-6 e domains of HN var2CSA was studied.Conclusions:This work represented the diversity of rar genes of a P.falciparum isolate in China.Objective:To analyse the rar gene repertoire and characterise the rhondroitin sulphate A (CSA)-binding activity of the Duffy-binding like(I)BI.) domains encoded by the var2csa gene of a Plasmodium falciparum(P.falciparum) isolate in Hainan Province,China.Methods:The sequences of var DBL1 regions were PCR-amplified,sequenced and the sequence characteristics was bioinformalically analysed.Recombinant proteins encoded by the var2csa genes were expressed and purified.The binding activities of the recombinant proteins to CSA receptor was detected by ELISA assays.Results:Fifty six unique DBI.a sequences were obtained,and the sequences represented similar diversity to the var genes of the genome parasite 3D7.There are two var2csa genes in the P.falciparum isolated from Hainan Province.Unlike in other falciparum parasites such as HB3,the two var2csa genes are more diverged.The receptor-binding capacity of DBL-5εand DBI.-6 e domains of HN var2CSA was studied.Conclusions:This work represented the diversity of rar genes of a P.falciparum isolate in China.

关 键 词：MALARIA PLASMODIUM FALCIPARUM Antigenic variation VAR gene 

分 类 号：R382[医药卫生—医学寄生虫学]