Larvicidal,ovicidal and pupicidal activities of Gliricidia sepium(Jacq.)(Leguminosae) against the malarial vector,Anopheles stephensi Liston(Culicidae:Diptera)  

作　　者：Kaliyamoorthy Krishnappa Shanmugam Dhanasekaran Kuppusamy Elumalai 

机构地区：[1]Unit of Entomotoxicity,Department of Zoology Govt.Thirumagal Mills College Vellore-632607 Tamilnadu,India [2]Center for Entomotoxicity Studies,Department of Zoology,Annamalai University,Annamalainagar-608 002 Tamilnadu,India

出　　处：《Asian Pacific Journal of Tropical Medicine》2012年第8期598-604,共7页亚太热带医药杂志（英文版）

基　　金：Higher Authorities for the grant of financial assistance with the Award of University Research Fellowship(Ref.No.No.H5/2009-2010/URF Order of the Vice chancellore,23011/2009);Professor and Head,Department of Zoology,Annamalai University for the laboratory facilities provided.

摘　　要：Objective:To investigate the potentiality of mosquitocidal activity of Gliricidia sepium（C.sepium） （Jacq.）（Leguminosae）.Methods:Twenty five early third instar larvae of Anopheles stephensi （An.stephensi） were exposed to various concentrations（50-250 ppm） and the 24 h LC₅₀ values of the G.sepium extract was determined by probit analysis.The ovicidal activity was determined against An.stephensi to various concentrations ranging from 25-100 ppm under laboratory conditions.The eggs hatchabilily was assessed 48 h post treatment.The pupicidal activity was determined against An.stephensi to various concentrations ranging from 25-100 ppm.Mortality of each pupa was recorded after 24 h of exposure to the extract.Results:Results pertaining to the experiment clearly revealed that ethanol extract showed significant larvicidal,ovicidal and pupicidal activity against the An.stephensi.Larvicidal activity of ethanol extracts of G.sepium showed maximum mortality in 250 ppm concentration（96.0±2.4）%.Furthermore,the LC₅₀ was found to be 121.79 and the LC₅₀ value was recorded to be 231.98 ppm.Ovicidal activity of ethanol extract was assessed by assessing the egg hatchabilily.Highest concentration of both solvent extracts exhibited 100%ovicidal activity.Similarly,pupae exposed to different concentratioiLs of ethanol extract were found dead with 58.10%adult emergence when it was treated with 25 ppm concentration.Similarly,18.36（n=30;61.20%）;21.28（70.93） and 27.33（91.10） pupal mortality was recorded from the experimental pupae treated with 50,75 and 100 ppm concentration of extracts. Three fractions have been tested for their larvicidal activity of which the Fraction 3 showed the LC₅₀ and LC₉₀ values of 23.23 and 40.39 ppm.With regard to the ovicidal effect fraction 3 showed highest ovicidal activities than the other two fractions.Furthermore,there were no hatchabilily was recorded above 50 ppm（100%egg mortality） in the experimental group.StatObjective:To investigate the potentiality of mosquitocidal activity of Gliricidia sepium(C.sepium) (Jacq.)(Leguminosae).Methods:Twenty five early third instar larvae of Anopheles stephensi (An.stephensi) were exposed to various concentrations(50-250 ppm) and the 24 h LC_(50) values of the G.sepium extract was determined by probit analysis.The ovicidal activity was determined against An.stephensi to various concentrations ranging from 25-100 ppm under laboratory conditions.The eggs hatchabilily was assessed 48 h post treatment.The pupicidal activity was determined against An.stephensi to various concentrations ranging from 25-100 ppm.Mortality of each pupa was recorded after 24 h of exposure to the extract.Results:Results pertaining to the experiment clearly revealed that ethanol extract showed significant larvicidal,ovicidal and pupicidal activity against the An.stephensi.Larvicidal activity of ethanol extracts of G.sepium showed maximum mortality in 250 ppm concentration(96.0±2.4)%.Furthermore,the LC_(50) was found to be 121.79 and the LC_(50) value was recorded to be 231.98 ppm.Ovicidal activity of ethanol extract was assessed by assessing the egg hatchabilily.Highest concentration of both solvent extracts exhibited 100%ovicidal activity.Similarly,pupae exposed to different concentratioiLs of ethanol extract were found dead with 58.10%adult emergence when it was treated with 25 ppm concentration.Similarly,18.36(n=30;61.20%);21.28(70.93) and 27.33(91.10) pupal mortality was recorded from the experimental pupae treated with 50,75 and 100 ppm concentration of extracts. Three fractions have been tested for their larvicidal activity of which the Fraction 3 showed the LC_(50) and LC_(90) values of 23.23 and 40.39 ppm.With regard to the ovicidal effect fraction 3 showed highest ovicidal activities than the other two fractions.Furthermore,there were no hatchabilily was recorded above 50 ppm(100%egg mortality) in the experimental group.Statistically significant pupicidal activity was recorded from 75 ppm concentration.

关 键 词：Anopheles STEPHENSI GLIRICIDIA sepium Crude extract Fractions LARVICIDAL ACTIVITY OVICIDAL ACTIVITY Pupicidal ACTIVITY 

分 类 号：S567.19[农业科学—中草药栽培]