Detection of STAT2 in early stage of cervical premalignancy and in cervical cancer  

作　　者：Liang Zeng Li-Hua Gao Li-Jun Cao De-Yun Feng Ya Cao Qi-Zhi Luo Ping Yu Ming Li 

机构地区：[1]Department of Pathology,Hunan Province Tumor Hospital [2]Department of Immunology,Basic Medical College,Central South University [3]Department of Anesthesiology,Second Xiangya Hospital [4]Department of Pathology,Basic Medical College,Central South University [5]Cancer Research Institute,Central South University

出　　处：《Asian Pacific Journal of Tropical Medicine》2012年第9期738-742,共5页亚太热带医药杂志（英文版）

基　　金：funded by 'Chinese National Nature Science Foundation(30771122)';'Chinese Ministry of Education,Research Start-up Funding for Home Smdents' to Ming Li

摘　　要：Objective:To measure the expression pattern of STAT2 in cenical cancer initiation and progression in tissue sections from patients with cervicitis,dysplasia,and cenical cancer. Methods:Antibody against human STAT2 was confirmed by plasmids transient transfection and Western blot Immunohistochemistry was used to delect STAT2 expression in the cervical biopsies by using the confirmed antibody against STAT2 as the primary antibody.Results:It was found that the overall rate of positive STAT2 expression in the cervicitis,dysplasia and cenical cancer groups were 38.5%,69.49%and 76.991,respectively.The STAT2 levels are significantly increased in premalignant dysplasia and cervical cancer,as compared lo cervicitis(P【 0.05). Noticeably,STAT2 signals were mainly found in the cytoplasm,implying that STAT2 was not biologically active.Conclusions:These findings reveal an association between cenical cancer progression and augmented STAT2 expression.In conclusion.STAT2 increase appears to be an early detectable cellular event in cenical cancer development.Objective:To measure the expression pattern of STAT2 in cenical cancer initiation and progression in tissue sections from patients with cervicitis,dysplasia,and cenical cancer. Methods:Antibody against human STAT2 was confirmed by plasmids transient transfection and Western blot Immunohistochemistry was used to delect STAT2 expression in the cervical biopsies by using the confirmed antibody against STAT2 as the primary antibody.Results:It was found that the overall rate of positive STAT2 expression in the cervicitis,dysplasia and cenical cancer groups were 38.5%,69.49%and 76.991,respectively.The STAT2 levels are significantly increased in premalignant dysplasia and cervical cancer,as compared lo cervicitis(P< 0.05). Noticeably,STAT2 signals were mainly found in the cytoplasm,implying that STAT2 was not biologically active.Conclusions:These findings reveal an association between cenical cancer progression and augmented STAT2 expression.In conclusion.STAT2 increase appears to be an early detectable cellular event in cenical cancer development.

关 键 词：CERVICAL cancer development IMMUNOHISTOCHEMISTRY STAT2 

分 类 号：R737.33[医药卫生—肿瘤]