Antibacterial activity of various honey types of Algeria against Staphylococcus aureus and Streptococcus pyogenes  

作　　者：Ahmed Moussa Djebli Noureddine Hammoudi Si Mohamed Meslem Abdelmelek Aissat Saad 

机构地区：[1]Institute of Veterinary Sciences University Ibn-khadoun Tiaret(14000),Algeria [2]Departments of Biology,Faculty of Sciences,Mostaganem University,Algeria

出　　处：《Asian Pacific Journal of Tropical Medicine》2012年第10期773-776,共4页亚太热带医药杂志（英文版）

摘　　要：Objective:To assess the in vitro antibacterial activity of honey from different geographical location on Gram negative organismes.Methods:Different concentrations(Undiluted honey,10%, 30%,50%and 70%wt/vol) of honey were studied in vitro using Staphylococcus aureus(S.aureus) and Streptococcus pyogenes(S.pyogenes),briefly,two-fold dilutions of honey solutions were tested to determine the minimum inhibitory concentration(MIC) against each type of microorganism,followed by more assays within a narrower dilution range to obtain more precise MIC values.MICs were determined by both visual inspection and spectrophotometric assay at 620 nm.These honey samples were compared with standard antibiotics like ampicillin,penicillin G,amoxicillin,gentamycin,tobramycin,erythromycin and chloramphenicol was determined by the disc diffusion method.Results:The diameter of zone of the inhibition(ZDI) of honey has various concentrations tested for the isolates ranged 0-46 mm for S.aureus,0-44 mm for S.pyogenes.While the MIC(%) ranged 12%-95%,25%-73%respectively.Conclusions:Algeria honey,in-vitro,possess antibacterial activity.Objective:To assess the in vitro antibacterial activity of honey from different geographical location on Gram negative organismes.Methods:Different concentrations(Undiluted honey,10%, 30%,50%and 70%wt/vol) of honey were studied in vitro using Staphylococcus aureus(S.aureus) and Streptococcus pyogenes(S.pyogenes),briefly,two-fold dilutions of honey solutions were tested to determine the minimum inhibitory concentration(MIC) against each type of microorganism,followed by more assays within a narrower dilution range to obtain more precise MIC values.MICs were determined by both visual inspection and spectrophotometric assay at 620 nm.These honey samples were compared with standard antibiotics like ampicillin,penicillin G,amoxicillin,gentamycin,tobramycin,erythromycin and chloramphenicol was determined by the disc diffusion method.Results:The diameter of zone of the inhibition(ZDI) of honey has various concentrations tested for the isolates ranged 0-46 mm for S.aureus,0-44 mm for S.pyogenes.While the MIC(%) ranged 12%-95%,25%-73%respectively.Conclusions:Algeria honey,in-vitro,possess antibacterial activity.

关 键 词：HONEY ANTIBACTERIAL activity STAPHYLOCOCCUS AUREUS STREPTOCOCCUS PYOGENES 

分 类 号：R515[医药卫生—内科学]