Antitubercular activity of the semi-polar extractives of Uvaria rufa  

作　　者：Allan Patrick G Macabeo Florie A Tudla Karsten Krohn Scott G Franzblau 

机构地区：[1]Organic Synthesis and Phytochemistry Laboratory,Research Center for the Natural Sciences,University of Santo Tomas,Espana Blvd.,Manila 1015,Philippines [2]Institut fr Organische Chemie,Universitat Regensburg,Universitatsstrasse 31,93053 Regensburg,Germany [3]Institut fr Organische Chemie,Universitat Paderborn,Germany [4]Institute for Tuberculosis Research,College of Pharmacy,University of Illinois.IL 60085,USA

出　　处：《Asian Pacific Journal of Tropical Medicine》2012年第10期777-780,共4页亚太热带医药杂志（英文版）

摘　　要：Objective:To investigate the inhibitory activity of the chloroform extract,petroleum ether and chloroform sub-extracts,lead-acetate treated chloroform extract,fractions and secondary metabolites of Uvaria rufa(U.rufa) against Mycobacterium tuberculosis(M.tuberculosis) H_(37)Rv. Methods:The antituberculosis susceptibility assay was earried out using the colorimetric Microplate Alamar blue assay(MABA).In addition,the cytotoxicity of the most active fraction was evaluated using the VERO cell toxicity assay.Results:The in vitro inhibitory activity against M.tuberculosis H_(37)Rv increased as purification progressed to fractionation(MIC up to 23μg/mL). The chloroform extract and its sub-extracts showed moderate toxicity while the most active fraction from chloroform sub-extract exhibited no cytotoxicity against VERO cells.Meanwhile, the lead acetate-treated crude chloroform extract and its fractions showed complete inhibitions (100%) with MIC values up to 8μg/mL.Phylochemical screening of the most active fraction showed,in general,the presence of terpenoids,steroids and phenolic compounds.Evaluation of the antimycobacterial activity of known secondary metabolites isolated showed no promising inhibitory activity against the test organism.Conclusions:The present results demonstrate the potential of U.rufa as a phytomedicinal source of compounds that may exhibit promising antituberculosis activity.In addition,elimination of polar pigments revealed enhanced inhibition against M.tuberculosis H_(37)Rv.While several compounds known for this plant did not show antimycobacterial activity,the obtained results are considered sufficient reason for further study to isolate the metabolites from U.rufa responsible for the antitubercular activity.Objective:To investigate the inhibitory activity of the chloroform extract,petroleum ether and chloroform sub-extracts,lead-acetate treated chloroform extract,fractions and secondary metabolites of Uvaria rufa（U.rufa） against Mycobacterium tuberculosis（M.tuberculosis） H₃₇Rv. Methods:The antituberculosis susceptibility assay was earried out using the colorimetric Microplate Alamar blue assay（MABA）.In addition,the cytotoxicity of the most active fraction was evaluated using the VERO cell toxicity assay.Results:The in vitro inhibitory activity against M.tuberculosis H₃₇Rv increased as purification progressed to fractionation（MIC up to 23μg/mL）. The chloroform extract and its sub-extracts showed moderate toxicity while the most active fraction from chloroform sub-extract exhibited no cytotoxicity against VERO cells.Meanwhile, the lead acetate-treated crude chloroform extract and its fractions showed complete inhibitions （100%） with MIC values up to 8μg/mL.Phylochemical screening of the most active fraction showed,in general,the presence of terpenoids,steroids and phenolic compounds.Evaluation of the antimycobacterial activity of known secondary metabolites isolated showed no promising inhibitory activity against the test organism.Conclusions:The present results demonstrate the potential of U.rufa as a phytomedicinal source of compounds that may exhibit promising antituberculosis activity.In addition,elimination of polar pigments revealed enhanced inhibition against M.tuberculosis H₃₇Rv.While several compounds known for this plant did not show antimycobacterial activity,the obtained results are considered sufficient reason for further study to isolate the metabolites from U.rufa responsible for the antitubercular activity.

关 键 词：Uvaria rufa ANTITUBERCULAR Mycobacterium tuberculosis H_(37)Rv 

分 类 号：R96[医药卫生—药理学]