Chemical composition and larvicidal effects of essential oil of Blumea martiniana against Anopheles anthropophagus  

作　　者：Liang Zhu Ying-juan Tian 

机构地区：[1]College of Food and Bioengineering,South China University of Technology,Guangzhou,Guangdong Province,China

出　　处：《Asian Pacific Journal of Tropical Medicine》2011年第5期371-374,共4页亚太热带医药杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China (50676041);financial support provided by the National Natural Science Foundation of China (20676041)

摘　　要：Objective:To determine chemical composition of the hydrodistilled essential oil of Blumea martiniana,and to evaluate its larvicidal activity against Anopheles anthropophagus,the natural vector of malaria.Methods:Chemical composition of essential oils extracted by steam distillation was investigated by gas chromatography and mass spectroscopy（GC-MS）.Larvicidal activity of essential oil and its four main compounds was carried out by WHO method.Twenty larvae of early fourth-instar stage were used in the larvicidal assay and five replicates were maintained for each concentration.The 24 h LC₅₀ and LC₉₀ values were determined following probit analysis.Results:Totally 68 compounds corresponding to 98.55%of the total oil were identified and the major constituents of essential oil were:linalool（10.36%）,germacrene D（9.09%）, borneol（6.24%）,γ-terpinene（5.38%）.The oil and linalool,germacrene D,bomeol,γ-terpinene exerted significant larvicidal activity with LC₅₀ values of 46.86,35.87,44.61,35.89,and 29.21 mg/L, respectively.It also showed a dose dependent effect on mortality.Conclusions:The essential oil of Blumea martiniana and its four major compounds may have potential for use in control of Anopheles anthropophagus larvae and could be useful in search of newer,safer and more effective natural compounds as larvicides.Objective:To determine chemical composition of the hydrodistilled essential oil of Blumea martiniana,and to evaluate its larvicidal activity against Anopheles anthropophagus,the natural vector of malaria.Methods:Chemical composition of essential oils extracted by steam distillation was investigated by gas chromatography and mass spectroscopy(GC-MS).Larvicidal activity of essential oil and its four main compounds was carried out by WHO method.Twenty larvae of early fourth-instar stage were used in the larvicidal assay and five replicates were maintained for each concentration.The 24 h LC_(50) and LC_(90) values were determined following probit analysis.Results:Totally 68 compounds corresponding to 98.55%of the total oil were identified and the major constituents of essential oil were:linalool(10.36%),germacrene D(9.09%), borneol(6.24%),γ-terpinene(5.38%).The oil and linalool,germacrene D,bomeol,γ-terpinene exerted significant larvicidal activity with LC_(50) values of 46.86,35.87,44.61,35.89,and 29.21 mg/L, respectively.It also showed a dose dependent effect on mortality.Conclusions:The essential oil of Blumea martiniana and its four major compounds may have potential for use in control of Anopheles anthropophagus larvae and could be useful in search of newer,safer and more effective natural compounds as larvicides.

关 键 词：Blumea martiniana ESSENTIAL OIL COMPOSITION LARVICIDAL ANOPHELES anthropophagus 

分 类 号：TQ453[化学工程—农药化工]