Effect of plumbagin free alcohol extract of Plumbago zeylanica Linn,root on reproductive system of female Wistar rats  

作　　者：Gupta Sandeep Ahirwar Dheeraj Sharma Neeraj Kumar Jhade Deenanath Ahirwar Bharti 

机构地区：[1]School of Pharmacy,Chouksey Engineering College,Bilaspur(C.G.),India 495001 [2]SLT Institute of Pharmaceutical Sciences,Guru Ghasidas University.Bilaspur(C.G.),India 495001

出　　处：《Asian Pacific Journal of Tropical Medicine》2011年第12期978-984,共7页亚太热带医药杂志（英文版）

摘　　要：Objective:To assess the effect of plumbagin-free alcohol extract（PFAE） of Plumbago zeylanica Linn.（Plumbaginaceae）（P.zeylanica） root,on female reproductive system and ferlility of adult Female wislar rats.Methods:After the oral acute toxicity study,the PFAE was administered at two dose levels to perform the estrous cycle study,anti-implantation and ahortifacient activity and hormonal analysis.However,the estrogenic/antiestrogenic activity was evaluated at only one most effective dose.Results:LD₅₀ cut-off was 5 000 mg/kg body weight.The extract exhibited significant anti-implantation and ahortifacient activity at the tested dose levels（300 and 500 mg/ kg.p.o.）（P【0.01）.The extract dose-dependency decreased the levels of serum progesterone, follicle stimulating hormone and luteinizing hormone,while a dose-dependent increase was observed in the concentration of serum prolactin.The extract did not show any significant changes in structure and function of Uterus when given alone,hut when given along with ethinyl estradiol,it exhibited significant antiestrogenic activity in immature overieclonzed female rats（P【0.001） Biochemical parameters in the serun/blood and haematological parameters did not show appreciable changes throughout and after the course of investigation.However,all the altered parameters returned to normalcy within 30 days following withdrawal of treatment. Conclusions:All findings suggest that the anlifcrtility activity of extract could possibly he through the changes in the implantation site,altered hormonal levels,prolonged estrous cycle and anti-estrogenic activity.Hence,the extract possesses reversible antifcrtility activity without adverse toxicity in female rats.Objective To assess the effect of plumbagin-free alcohol extract (PFAE) of Plumbago zeylanica Linn. (Plumbaginaceae) (P. zeylanica) root, on female reproductive system and fertility of adult female wistar rats. Methods After the oral acute toxicity study, the PFAE was administered at two dose levels to perform the estrous cycle study, anti-implantation and abortifacient activity and hormonal analysis. However, the estrogenic/antiestrogenic activity was evaluated at only one most effective dose. Results LD50 cut-off was 5 000 mg/kg body weight. The extract exhibited significant anti-implantation and abortifacient activity at the tested dose levels (300 and 500 mg/kg, p.o.) (P<0.01). The extract dose-dependently decreased the levels of serum progesterone, follicle stimulating hormone and luteinizing hormone, while a dose-dependent increase was observed in the concentration of serum prolactin. The extract did not show any significant changes in structure and function of uterus when given alone, but when given along with ethinyl estradiol, it exhibited significant antiestrogenic activity in immature overiectomized female rats(P<0.001). Biochemical parameters in the serum/blood and haematological parameters did not show appreciable changes throughout and after the course of investigation. However, all the altered parameters returned to normalcy within 30 days following withdrawal of treatment. Conclusions All findings suggest that the antifertility activity of extract could possibly be through the changes in the implantation site, altered hormonal levels, prolonged estrous cycle and anti-estrogenic activity. Hence, the extract possesses reversible antifertility activity without adverse toxicity in female rats.

关 键 词：ANTI-IMPLANTATION Abortilacient Estrogenic/antiestrogenic activity Progesterone Follicle stimulating HORMONE Luteinizing HORMONE PROLACTIN Ethinyl ESTRADIOL Overiectomized Haematological 

分 类 号：R285[医药卫生—中药学]