Use of reducing agents for the aerobic growth of Campylobacter jejuni  

作　　者：Rabia Sultana Siddiqui Siti Asma H 

机构地区：[1]School of Dental Sciences,Universiti Sains Malaysia,16150 Kubang Kerian,Kelantan,Malaysia [2]School of Medical Sciences,Universiti Sains Malaysia,16150 Kubang Kerian,Kelantan,Malaysia

出　　处：《Asian Pacific Journal of Tropical Medicine》2010年第3期235-236,共2页亚太热带医药杂志（英文版）

摘　　要：Objective:To produce a technique for the growth of Campylobacter jejuni in aerobic condition Methods:Different combinations of reducing agents were tested in brucella broth and the growth turbidity was compared with tubes containing normal broth only.Microaerophilic environment was also provided in a petri plate seeded with Campylobacter culture by pouring 3 different concentrations（10%,5%and 2.5%） of five reducing agents along with bacto-agar in the lid which was used to cover and seal the culture plate.Six reducing agents were also added in broth in concentration of 0.25 mg/mL of each with different combinations.Results:In lid agar technique,Campylobacter jejuni growth appeared in all three concentrations of reducing agents, that is 10%,5%and 2.5%after 24 hours of incubation but the best results were observed in 10% concentration.The colonial and morphological characters were not affected when the organisms were grown by this technique.Conclusions:It was found that reducing agents enhance the growth of C.jejuni/coli.In combination of FeSO₄,Na₂CO₃ with H₃BO₃ worked as ideal mixture for the aerobic growth of Campylobacter.This technique is more economical as compared to commercially available media in the market and can be used for the oral facultative and microaerophilic bacterial growth in laboratory.Objective:To produce a technique for the growth of Campylobacter jejuni in aerobic condition Methods:Different combinations of reducing agents were tested in brucella broth and the growth turbidity was compared with tubes containing normal broth only.Microaerophilic environment was also provided in a petri plate seeded with Campylobacter culture by pouring 3 different concentrations(10%,5%and 2.5%) of five reducing agents along with bacto-agar in the lid which was used to cover and seal the culture plate.Six reducing agents were also added in broth in concentration of 0.25 mg/mL of each with different combinations.Results:In lid agar technique,Campylobacter jejuni growth appeared in all three concentrations of reducing agents, that is 10%,5%and 2.5%after 24 hours of incubation but the best results were observed in 10% concentration.The colonial and morphological characters were not affected when the organisms were grown by this technique.Conclusions:It was found that reducing agents enhance the growth of C.jejuni/coli.In combination of FeSO_4,Na_2CO_3 with H_3BO_3 worked as ideal mixture for the aerobic growth of Campylobacter.This technique is more economical as compared to commercially available media in the market and can be used for the oral facultative and microaerophilic bacterial growth in laboratory.

关 键 词：Camylobacter JEJUNI MICROAEROPHILIC REDUCING AGENTS LID AGAR technique 

分 类 号：R378[医药卫生—病原生物学]