Brazilian vaccinia virus strains show a classical orthopoxvirus infection course and cross-protection  

作　　者：Jaqueline Maria Siqueira Ferreira Betnia Paiva Drumond J?natas Santos Abraho Zélia Inês Portela Lobato Cláudio Ant?nio Bonjardim Paulo César Peregrino Ferreira Erna Geessien Kroon 

机构地区：Laboratorio de Virus,Departamento de Microbiologia,Instituto de Ciencias Biologicas Departamento de Medicina Veterinaria Preventiva,Escola de Veterindria,Universidade Federal de Minas Gerais.Avenida Antonio Carlos,6627,caixa postal 486,CEP 31270-901,Belo Horizonte,MG,Brazil

出　　处：《Asian Pacific Journal of Tropical Medicine》2009年第2期21-26,共6页亚太热带医药杂志（英文版）

基　　金：Financial support was provided by CNPq, CAPES and FAPEMIG.Japueline M.S.Ferreira received fellowship from CAPES. R.M.S.Ferreira received fellowship from CAPES.R.M.E. P.C.P. Ferreira, C.A.Bonjardim,Z.I.P. Lobato and E.G.Kroon are recipients of research fellowship from CNPq

摘　　要：Objectives:The purpose of this work was to study the infection course and cross-protection in mice after intradermal injection of Vaccinia virus(VACV) strain Western Reserve and three Brazilian VACV strains: Aracatuba,Muriae and BeAn58058 isolated from cow,human and rodent,respectively.Methods:Balb/c mice were inoculated by footpad and back scarification and daily monitored regarding lesion development and weight loss.To check cross protection after intradermal VACV inoculation,mice were subsequendy infected with different VACV strains and monitored to check lesion development.Serum neutralization assays were performed to check for the presence of antibodies against Orthopoxvirus.Results:After VACV intradermal inoculation the lesion development pattern was similar in mice infected with the different virus strains.By using the footpad scarification model,cross-protection among VACV strains was observed.Moreover,neutralizing antibodies against Orthopoxvirus were detected in sera from mice infected with all VACV strains.Conclusion:Although it was not possible to observe virulence differences among VACV strains isolated from cow,rodent and human using the murine model,this inoculation route showed to be an appropriated model to study lesions development since it mimics natural infections by VACV in nature. <正>Objectives:The purpose of this work was to study the infection course and cross-protection in mice after intradermal injection of Vaccinia virus(VACV) strain Western Reserve and three Brazilian VACV strains: Aracatuba,Muriae and BeAn58058 isolated from cow,human and rodent,respectively.Methods:Balb/c mice were inoculated by footpad and back scarification and daily monitored regarding lesion development and weight loss.To check cross protection after intradermal VACV inoculation,mice were subsequendy infected with different VACV strains and monitored to check lesion development.Serum neutralization assays were performed to check for the presence of antibodies against Orthopoxvirus.Results:After VACV intradermal inoculation the lesion development pattern was similar in mice infected with the different virus strains.By using the footpad scarification model,cross-protection among VACV strains was observed.Moreover,neutralizing antibodies against Orthopoxvirus were detected in sera from mice infected with all VACV strains.Conclusion:Although it was not possible to observe virulence differences among VACV strains isolated from cow,rodent and human using the murine model,this inoculation route showed to be an appropriated model to study lesions development since it mimics natural infections by VACV in nature.

关 键 词：ORTHOPOXVIRUS ZOONOSES Balb/c mice INTRADERMAL injection VACCINIA virus Bovine VACCINIA outbreaks 

分 类 号：R392[医药卫生—免疫学]