Phytopharmacological evaluation of ethanol extract of Sida cordifolia L.roots  

作　　者：Mohammad Abdul Motalib Momin Sm Faysal Bellah Sarder Mohammad Raussel Rahman Ahmed Ayedur Rahman Gazi Mohammad Monjur Murshid Sarder Mohammad Saker Billah Talha Bin Emran 

机构地区：[1]Department of Pharmacy,Jahangirnagar University [2]Department of Clinical Pharmacy and Pharmacology,Dhaka University [3]Pharmacy Discipline,Life Science School,Khulna University [4]Department of Chemistry,Gort.M.M.University College [5]Department of Pharmacy,BGC Trust University

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2014年第1期18-24,共7页亚太热带生物医学杂志（英文版）

基　　金：Supported by the Ministry of National Science,Information and Communication Technology(NSICT)of People's Republic of Bangladesh(Grant No.1259/04)

摘　　要：Objective:To investigate the phytochemical screening(group determination)and selected pharmacological activities(antioxidant,antimicrobial and analgesic activity)of the plant Sida cordifolia Linn(S.cordifolia).Methods:Eighty percent concentrated ethanol extract of the roots was used.To identify the chemical constituents of plant extract standard procedures were followed.In phytochemical screening the crude extract was tested for the presence of different chemical groups like reducing sugar,tannins,saponins,steroids,flavonoids,gums,alkaloids and glycosides.The antioxidant property of ethanolic extract of S.cordifolia was assessed by DPPH free radical scavenging activity.Analgesic activity of the extract was tested using the model of acetic acid induced writhing in mice.Diclofenac sodium is used as reference standard drug for the analgesic activity test Antibacterial activity of plant extract was carried out using disc diffusion method with five pathogenic bacteria comparison with kanamycin as a standard.Results:Phytochemical analysis of the ethanolic extract of the roots of S.cordifolia indicated the presence of reducing sugar,alkaloids,steroids and saponins.In DPPH scavenging assay the IC_(50)value was found to be 50 ug/mL which was not comparable to the standard ascorbic acid.The crude extract produced 44.3%inhibition of writhing at the dose of 300 mg/kg body weight which is statistically significant(P>0.001).The in vitro antimicrobial activity of the ethanol extract of the roots of S.cordifolia showed no antimicrobial activity against five types of microorganisms.The experiment was conducted only with five species of bacteria as test species,which do not at all indicate the total inactivity against micro-organisms.Condusions:The obtained results provide a support for the use of this plant in traditional medicine but further pharmacological studies are required.Objective:To investigate the phytochemical screening（group determination）and selected pharmacological activities（antioxidant,antimicrobial and analgesic activity）of the plant Sida cordifolia Linn（S.cordifolia）.Methods:Eighty percent concentrated ethanol extract of the roots was used.To identify the chemical constituents of plant extract standard procedures were followed.In phytochemical screening the crude extract was tested for the presence of different chemical groups like reducing sugar,tannins,saponins,steroids,flavonoids,gums,alkaloids and glycosides.The antioxidant property of ethanolic extract of S.cordifolia was assessed by DPPH free radical scavenging activity.Analgesic activity of the extract was tested using the model of acetic acid induced writhing in mice.Diclofenac sodium is used as reference standard drug for the analgesic activity test Antibacterial activity of plant extract was carried out using disc diffusion method with five pathogenic bacteria comparison with kanamycin as a standard.Results:Phytochemical analysis of the ethanolic extract of the roots of S.cordifolia indicated the presence of reducing sugar,alkaloids,steroids and saponins.In DPPH scavenging assay the IC50value was found to be 50 ug/mL which was not comparable to the standard ascorbic acid.The crude extract produced 44.3%inhibition of writhing at the dose of 300 mg/kg body weight which is statistically significant（P>0.001）.The in vitro antimicrobial activity of the ethanol extract of the roots of S.cordifolia showed no antimicrobial activity against five types of microorganisms.The experiment was conducted only with five species of bacteria as test species,which do not at all indicate the total inactivity against micro-organisms.Condusions:The obtained results provide a support for the use of this plant in traditional medicine but further pharmacological studies are required.

关 键 词：Antioxidant ANTIMICROBIAL ANALGESIC DPPH PHYTOCHEMICAL screening 

分 类 号：R285[医药卫生—中药学]