Modulatory effects of dietary inclusion of garlic(Allium sativum) on gentamycin-induced hepatotoxicity and oxidative stress in rats  

作　　者：Adedayo O Ademiluyi Ganiyu oboh Tosin R Owoloye Oluwaseun J Agbebi 

机构地区：[1]Functional Foods,Nutraceuticals and Phytomedicine Unit,Department of Biochemistry,Federal University of Technology

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2013年第6期470-475,共6页亚太热带生物医学杂志（英文版）

基　　金：Supported by Federal University of Technology,Akure Staff Development Grant No.VCPU/URGC/46

摘　　要：Objective:To investigate die ameliorative effect of dietary inclusion of garlic(Allium sativum)on gentamycin-induced hepatotoxicity in rats.Methods:Adult male rats were randomly divided into four groups with sis animals in each group.Groups 1 and 2 were fed basal diet while Groups 3 and 4 were fed diets containing 2%and 4%garlic respeetively for 27 d prior to gentamycin administration.Hepatotoxicity was induced by the intraperitoneal administration at gentamycin(100 mg/kg body weight)for 3 d.The liver and plasma were studied for hepatotoxicity and antioxidant indices.Results:Gentamycin induces hepatic damage as revealed by significant(P<0.05)elevation of liver damage marker enzymes(aspartate transaminase and alanine aminotransferase)and reduction in plasma albumin level.Gentamycin also eaused a significant(P<0.05)alteration in plasma and liver enzymatic(catalase,glutathione and super oxygen dehydrogenises)and non-enzymatic(glutathione and vitamin C)antioxidant indices with concomitant increase in the malondialdehyde content;however,there was a significant(P<0.05)restoration of the antioxidant status coupled with significant(P<0.05)decrease in the tissues malondialdehyde content,following consumption of diets containing garlic.Conclusions:These results suggest that dietary inclusion of garlic powder could protect against gentamycin-induced hepatotoxicity,improve antioxidant status and modulate oxidative stress;a function attributed to their phenolic constituents.Objective:To investigate die ameliorative effect of dietary inclusion of garlic(Allium sativum)on gentamycin-induced hepatotoxicity in rats.Methods:Adult male rats were randomly divided into four groups with sis animals in each group.Groups 1 and 2 were fed basal diet while Groups 3 and 4 were fed diets containing 2%and 4%garlic respeetively for 27 d prior to gentamycin administration.Hepatotoxicity was induced by the intraperitoneal administration at gentamycin(100 mg/kg body weight)for 3 d.The liver and plasma were studied for hepatotoxicity and antioxidant indices.Results:Gentamycin induces hepatic damage as revealed by significant(P&lt;0.05)elevation of liver damage marker enzymes(aspartate transaminase and alanine aminotransferase)and reduction in plasma albumin level.Gentamycin also eaused a significant(P&lt;0.05)alteration in plasma and liver enzymatic(catalase,glutathione and super oxygen dehydrogenises)and non-enzymatic(glutathione and vitamin C)antioxidant indices with concomitant increase in the malondialdehyde content;however,there was a significant(P&lt;0.05)restoration of the antioxidant status coupled with significant(P&lt;0.05)decrease in the tissues malondialdehyde content,following consumption of diets containing garlic.Conclusions:These results suggest that dietary inclusion of garlic powder could protect against gentamycin-induced hepatotoxicity,improve antioxidant status and modulate oxidative stress;a function attributed to their phenolic constituents.

关 键 词：GENTAMYCIN HEPATOTOXICITY Oxidative stress Garlic(Allium sativum) PHYTOCHEMICALS 

分 类 号：R285.5[医药卫生—中药学]