Saponins-rich fraction of Calotropis procera leaves elicit no antitrypanosomal activityin a rat model  

作　　者：Mohammed Auwal Ibrahim Abubakar Babando Aliyu Kayode Meduteni Isa Yunusa 

机构地区：[1]Departments of 1Biochemistry,Zaria, Nigeria [2]Departments of Chemistry, Ahmadu Bello University,Zaria, Nigeria [3]Department of Biochemistry, Kano University of Science and Technology,Wudil, Nigeria

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2013年第7期569-572,共4页亚太热带生物医学杂志（英文版）

基　　金：This study was supported,in part,by the Education Trust FundABU desk office with reference ETF/DESS/AST&D/ABU ZARIA

摘　　要：Objective:To examine thein vitroandin vivoanti-Trypanosoma evansi(T.evansi)activity ofsaponins-rich fraction ofCalotropis procera(cpsf)leaves as well as the effect of the fraction onthe parasite-induced anemia.Methods:A 60-minutes time course experiment was conductedwith various concentrations of the fraction using a 96-well microtiter plate technique,andsubsequently used to treat experimentallyT.evansiinfected rats at 100 and 200 mg/kg bodyweight.Index of anemia was analyzed in all animals during the experiment.Results:The cpsfdid not demonstrate anin vitroantitrypanosomal activity.Further,the cpsf treatments did notsignificantly(P>0.05)keep the parasites lower than the infected untreated groups.At the end ofthe experiment,allT.evansiinfected rats developed anemia whose severity was not significantly(P>0.05)ameliorated by the cpsf treatment.Conclusions:It was concluded that saponins derivedfromCalotropis proceraleaves could not elicitin vitroandin vivoactivities againstT.evansi.Objective:To examine thein vitroandin vivoanti-Trypanosoma evansi(T.evansi)activity ofsaponins-rich fraction ofCalotropis procera(cpsf)leaves as well as the effect of the fraction onthe parasite-induced anemia.Methods:A 60-minutes time course experiment was conductedwith various concentrations of the fraction using a 96-well microtiter plate technique,andsubsequently used to treat experimentallyT.evansiinfected rats at 100 and 200 mg/kg bodyweight.Index of anemia was analyzed in all animals during the experiment.Results:The cpsfdid not demonstrate anin vitroantitrypanosomal activity.Further,the cpsf treatments did notsignificantly(P>0.05)keep the parasites lower than the infected untreated groups.At the end ofthe experiment,allT.evansiinfected rats developed anemia whose severity was not significantly(P>0.05)ameliorated by the cpsf treatment.Conclusions:It was concluded that saponins derivedfromCalotropis proceraleaves could not elicitin vitroandin vivoactivities againstT.evansi.

关 键 词：ANEMIA ASCLEPIADACEAE Calotropis procera SAPONINS TRYPANOSOMA evansi 

分 类 号：S852.7[农业科学—基础兽医学]