Identification of an anticancer compound against HT-29 cells from Phellinus linteus grown on germinated brown rice  

作　　者：Tae-Il Jeon Chang-Hwa Jung Jeong-Yong Cho Dong Ki Park Jae-Hak Moon 

机构地区：[1]Department of Animal Science.College of Agriculture & Life Science,Chonnam National University,Korea [2]Food Function Research Center,Korea Food Research Institute,Seoungnam,Korea [3]Division of Food Biotechnology,University of Science and Technology,Daejeon,Korea [4]Department of Food Science & Technology,and Functional Food Research Center.Chonnam National University,Korea [5]Department of Bioscience and Biotechnology,Konkuk University,Korea

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2013年第10期785-789,共5页亚太热带生物医学杂志（英文版）

基　　金：Supported by a grant from the Korea Food Research Institute(Grant number:E0131601)

摘　　要：Objective:To isolate and identify the anticancer compound against proliferation of human colon cancer cells from ethyl acetate(EtOAc)extract ol Phellinus linteus grown on germinated brown rice(PB).Methods:EtOAc extract of PB was partitioned with n-hexane,EtOAc,and water-saturated n-butanol.Anticancer compound of n-hexane layer was isolated and identified by HPLC and NMR,respectively.Cytotoxicity against HT-29 cells was tested by SRB assay.Results:The n-hexane layer obtained after solvent fractionation of PB EtOAc extracts showed a potent anticancer activity against the HT-29 cell line.Atractylenolide I,a eudesmane-type sesquiterpene lactone,a major anticancer substance of PB,was isolated from the n-hexane layer by silica gel column chromatography and preparative-HPLC.This structure was elucidated by one-and two-dimensional NMR spectroscopic data.Atractylenolide I has not been reported in mushrooms or rice as of yet.The isolated compound dose-dependently inhibited the growth of HT-29 human colon cancer cells.Conclusions:Atractylenolide I might contribute to the anticancer effect of PB.Objective:To isolate and identify the anticancer compound against proliferation of human colon cancer cells from ethyl acetate(EtOAc)extract ol Phellinus linteus grown on germinated brown rice(PB).Methods:EtOAc extract of PB was partitioned with n-hexane,EtOAc,and water-saturated n-butanol.Anticancer compound of n-hexane layer was isolated and identified by HPLC and NMR,respectively.Cytotoxicity against HT-29 cells was tested by SRB assay.Results:The n-hexane layer obtained after solvent fractionation of PB EtOAc extracts showed a potent anticancer activity against the HT-29 cell line.Atractylenolide I,a eudesmane-type sesquiterpene lactone,a major anticancer substance of PB,was isolated from the n-hexane layer by silica gel column chromatography and preparative-HPLC.This structure was elucidated by one-and two-dimensional NMR spectroscopic data.Atractylenolide I has not been reported in mushrooms or rice as of yet.The isolated compound dose-dependently inhibited the growth of HT-29 human colon cancer cells.Conclusions:Atractylenolide I might contribute to the anticancer effect of PB.

关 键 词：Atractylenolide I Human COLON cancer cells NMR PHELLINUS linteus Germinated BROWN RICE 

分 类 号：TS201.2[轻工技术与工程—食品科学] R285[轻工技术与工程—食品科学与工程]