Production of asiaticoside from centella(Centella asiatica L.Urban)cells in bioreactor  

作　　者：Nguyen Hoang Loc Nguyen Thi Duy Nhat 

机构地区：[1]Institute of Resources.Environment and Biotechnology.Hue University,Hue 47000,Vietnam

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2013年第10期806-810,共5页亚太热带生物医学杂志（英文版）

基　　金：supported by the National Foundation for Science and Technology Development(NAFOSTED)of Vietnam(Grant No.106.16-2012.80)

摘　　要：Objective:To investigate the effects of some culture conditions on production of asialicoside from centella(Centella asiatica L.Urban)cells cultured in 5-L bioreactor.Methods:The centell cell suspension culture was conducted in 5-L bioreactor to investigate the growth and asiaticoside accumulation under various conditions.Asiaticoside content was determined by HPLC analysis.Results:The results showed that the cell growth and asiaticoside accumulation peaked after 24d of culture at an agitation speed of 150 r/min and aeration rate of 2.5 L/min.The cell biomass reached a maximum value of 302.45 g fresh weight(31.43 g dry weight)and growth index of 3.03with inoculum size of 100 g.However,asiaticoside content was the highest(60.08 mg/g dry weight)when culture was initiated with an inoculum size of 50 g.Conclusions:The present study found the suitable conditions for growth of centella cells and their asiaticoside production in bioreactor.Objective:To investigate the effects of some culture conditions on production of asialicoside from centella(Centella asiatica L.Urban)cells cultured in 5-L bioreactor.Methods:The centell cell suspension culture was conducted in 5-L bioreactor to investigate the growth and asiaticoside accumulation under various conditions.Asiaticoside content was determined by HPLC analysis.Results:The results showed that the cell growth and asiaticoside accumulation peaked after 24d of culture at an agitation speed of 150 r/min and aeration rate of 2.5 L/min.The cell biomass reached a maximum value of 302.45 g fresh weight(31.43 g dry weight)and growth index of 3.03with inoculum size of 100 g.However,asiaticoside content was the highest(60.08 mg/g dry weight)when culture was initiated with an inoculum size of 50 g.Conclusions:The present study found the suitable conditions for growth of centella cells and their asiaticoside production in bioreactor.

关 键 词：ASIATICOSIDE BIOREACTOR CENTELLA asiatica Cell SUSPENSION CULTURE 

分 类 号：TQ461[化学工程—制药化工]