Rac1 activates HIF-1 in laser induced choroidal neovascularization  

作　　者：Peng Zhang, Jian Zhou 

机构地区：[1]Department of Ophthalmology, Xijing Hospital, Fourth Military Medical University, Xi'an710032, Shaanxi Province, China [2]Drug Research and Development Center, Preclinical Medicine School, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China [3]Eye Hospital of China Academy of Traditional Chinese Medicine, Beijing 100040, China

出　　处：《International Journal of Ophthalmology(English edition)》2011年第1期14-18,共5页国际眼科杂志（英文版）

基　　金：National Natural Science Foundation of China(No.30371516, 30672291);Alexander Von Humbonldt Foundation in Germany (to YS Wang, V8151/02085)

摘　　要：AIM: To study the effect of Rac1 on the induction of HIF-1 alpha in choroidal neovascularization (CNV) in mice. METHODS: One hundred C57BL/6J mice were laser photocoagulated to induce CNV, fifty mice of that were selected randomly for intravitreal injection of Rac1 inhibitor NSC23766 solution (1 mu L). After laser photocoagulation, fundus fluorescein angiography (FFA) was performed to verify the growth of CNV. Immunohistochemistry and Western blot were used to detect HIF-1 alpha and Rac1 in posterior segment of eye globes. RESULTS: FFA verified that incidence of CNV was significantly reduced in the eyes with NSC23766 injection comparing with that of eyes without NSC23766 injection (P< 0.01). Immunohistochemistry detected that HIF-1 alpha and Rac1 mainly Expressed in the new fibrovascular tissue. Western blot showed that HIF-1 alpha and Rac1 was highly increased in tissue explants of retinal pigment epithelium (RPE) and choroid without NSC23766 injection. But for tissue explants of RPE and choroid with N5C23766 injection, both the expressions of HIF-1 alpha and Rac1 were inhibited. CONCLUSION: Rac1 is crucial to activate HIF-1 regulating the growth of CNV, and its inhibition may have potential therapeutic value.AIM: To study the effect of Rac1 on the induction of HIF-1 alpha in choroidal neovascularization (CNV) in mice. METHODS: One hundred C57BL/6J mice were laser photocoagulated to induce CNV, fifty mice of that were selected randomly for intravitreal injection of Rac1 inhibitor NSC23766 solution (1 mu L). After laser photocoagulation, fundus fluorescein angiography (FFA) was performed to verify the growth of CNV. Immunohistochemistry and Western blot were used to detect HIF-1 alpha and Rac1 in posterior segment of eye globes. RESULTS: FFA verified that incidence of CNV was significantly reduced in the eyes with NSC23766 injection comparing with that of eyes without NSC23766 injection (P< 0.01). Immunohistochemistry detected that HIF-1 alpha and Rac1 mainly Expressed in the new fibrovascular tissue. Western blot showed that HIF-1 alpha and Rac1 was highly increased in tissue explants of retinal pigment epithelium (RPE) and choroid without NSC23766 injection. But for tissue explants of RPE and choroid with N5C23766 injection, both the expressions of HIF-1 alpha and Rac1 were inhibited. CONCLUSION: Rac1 is crucial to activate HIF-1 regulating the growth of CNV, and its inhibition may have potential therapeutic value.

关 键 词：animal study choroidal neovascularisation growth factor signal transduction molecular biology 

分 类 号：R773.4[医药卫生—眼科]