Comparison of FGFR1 expression on lens epithelial cells between adults and fetuses  

作　　者：Yu-Fu Liu, Shu-Ling Peng 

机构地区：[1]Beijing Ophthalmology & Visual Sciences Key Lab, Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing 10005, China [2]Beijing Institute of Ophthalmology, Beijing 100730, China

出　　处：《International Journal of Ophthalmology(English edition)》2011年第1期37-39,共3页国际眼科杂志（英文版）

摘　　要：AIM: To study the differences of fibroblast growth factor receptor 1 (FGFR1) gene on human lens epithelial cells (HLECs) of adults and fetuses. METHODS: Indirect in situ RT-PCR was adopted for detection of FGFR1 gene. The cDNA of the nnRNA in the paraffin sections of fetus and adult HLEC was synthesized by reverse transcription reaction. After PCR amplification, in situ hybridization test was performed with synthesized oligonucleotide probe and relative quantification was carried out using image analysis. RESULTS: HLECs of adults and fetuses expressed FGFR1 gene, the expression level was higher in fetuses than in adults. The difference between them had significance (P<0.05). CONCLUSION: FGFR1 Exist in HLEC and the expression is age-related, which could be one of causes of the high occurrence of post operational after-cataract in children.AIM: To study the differences of fibroblast growth factor receptor 1 (FGFR1) gene on human lens epithelial cells (HLECs) of adults and fetuses. METHODS: Indirect in situ RT-PCR was adopted for detection of FGFR1 gene. The cDNA of the nnRNA in the paraffin sections of fetus and adult HLEC was synthesized by reverse transcription reaction. After PCR amplification, in situ hybridization test was performed with synthesized oligonucleotide probe and relative quantification was carried out using image analysis. RESULTS: HLECs of adults and fetuses expressed FGFR1 gene, the expression level was higher in fetuses than in adults. The difference between them had significance (P<0.05). CONCLUSION: FGFR1 Exist in HLEC and the expression is age-related, which could be one of causes of the high occurrence of post operational after-cataract in children.

关 键 词：human lens epithelial cells fibroblast growth factor receptor 1 indirect in situ RT-PCR AFTER-CATARACT 

分 类 号：R77[医药卫生—眼科]