翘嘴鳜线粒体DNA细胞色素b基因序列分析  

Sequence analysis of the coding region in mitochondrial cytochrome b gene from Siniperca chuatsi

作　　者：陈艺燕[1] 钱开诚[1] 章群[1] 

机构地区：[1]暨南大学水生生物研究所,中国广州510632

出　　处：《生态科学》2004年第2期154-155,159,共3页Ecological Science

摘　　要：测定了翘嘴鳜(Siniperca chuatsi)的线粒体细胞色素b基因编码区全序列1140个核苷酸序列,并且由此推导出对应的氨基酸序列,A/T所占比例为53.4%,G+C约占46.6%。该基因中密码子第1位核苷酸中4种碱基组成较为均衡,第2位核苷酸中T的使用率较高,G的使用率较低;而密码子第3位C/A的使用率高,而G的使用率仅为3.7%;在氨基酸序列中Leu所占比例16.36%,远高于其它氨基酸;而Cys使用比例仅为0.79%。本研究为鳜类的系统发育、资源保护和利用提供了核苷酸序列方面的资料,至于翘嘴鳜线粒体细胞色素b基因序列中密码子不同位置碱基使用比率以及氨基酸组成是翘嘴鳜特有还是鳜类共有特征,尚有待进一步研究。nucleotides of the coding region have been sequenced in the mitochondrial cytochrome b gene from Mandarin fish S. chuatsi and the corresponding amino acid sequence has also been deduced. The contents of A+T and G+C are 53.4% and 46.6% respectively。While the first positions of the codons have more or less even usage in 4 nucleotides, the second positions of the codons in the gene have bias to higher T and lower G;and only 3.7% G were found in the third positions. In deduced amino acid sequences, the percentage of Leu used in cytb gene were over twice than any other amino acids, the usage of Cys accounted for only 0.79%. Further studies will determine whether the nucleotide usage in different positions of codons and composition of amino acids were characteristic of S. chuatsi or sinipercinae, and molecular phylogeny and phylogeography of Sinipercinid fishes.

关 键 词：翘嘴鳜 线粒体 细胞色素B 基因 序列分析 密码子 

分 类 号：Q959.483[生物学—动物学] Q173