罗格列酮对帕金森病模型小鼠炎症反应和细胞凋亡的影响  

Influence of Rosiglitazone on inflammatory response and apoptosis in mouse model with Parkinson's disease

作　　者：张田[1] 魏子峰[2] 秦丽娟[1] 周洪霞[2] 张宇新[2] 

机构地区：[1]河北联合大学基础医学院生理学教研室,河北唐山063000 [2]河北联合大学基础医学院解剖学教研室

出　　处：《中国老年学杂志》2014年第9期2480-2482,共3页Chinese Journal of Gerontology

基　　金：河北省自然科学基金(No.C2004000689);河北省博士基金(No.05547008D-4);河北省科学技术与社会发展计划(No.04276135);唐山市科学技术研究与发展计划(No.10140201A-15)

摘　　要：目的观察炎症反应和细胞凋亡在帕金森病发病过程中的作用,探讨罗格列酮对PD多巴胺(DA)能神经元的保护作用。方法采用1-甲基-4-苯基-1,2,3,6四氢吡啶(MPTP)制备PD模型,观察各组小鼠行为学变化,免疫组织化学和免疫印迹观察小鼠中脑黑质酪氨酸羟化酶(TH)、环氧合酶(COX)-2、前列腺素E2(PGE2)和半胱氨酸蛋白酶(caspase)-3的表达变化,及给予罗格列酮后对上述变化的影响。结果模型组小鼠出现震颤、步态迟缓等PD样症状,黑质区TH阳性神经元缺失,COX-2、PGE2和caspase-3阳性细胞明显增多,蛋白水平亦升高;经罗格列酮治疗后,上述症状得到改善。结论罗格列酮对PD小鼠DA能神经元保护作用可能与抑制炎症反应和细胞凋亡有关。Objective To investigate the effects of inflammatory response and apoptosis during the pathogenetic process of Parkin -son′s disease (PD) and explore the protective effect of Rosiglitazone on dopaminergic neurons in PD.Methods C57BL/6N mice were ad-ministrated with MPTP to produce subacute PD model.The behavioral changes of different groups were observed , immunohistochemistry and Western blot methods were used to investigate the positive cell numbers and the protein expression of COX -2, PGE2, caspase-3 and TH.The above changes , after giving Rosiglitazone , were also studied.Results The model mice exhibited typical PD-like behaviors , TH-positive neurons lost markedly , the number of COX-2, PGE2, caspase-3 and TH immunoreactive cells and their expression level in SN of the mid-brain increased markedly.After giving Rosiglitazone , the above changes were reduced obviously.Conclusions The neuroprotective effect of Rosiglitazone on the dopaminergic neurons may inhibit the inflammatory response and apoptosis .

关 键 词：帕金森病 酪氨酸羟化酶 环氧合酶-2 前列腺素E2 半胱氨酸蛋白酶-3 罗格列酮 

分 类 号：R745[医药卫生—神经病学与精神病学]