miR-342-3p对三阴性乳腺癌化疗敏感性的影响  被引量:8

Effect of miR-342-3p on chemotherapy sensitivity in triple-negative breast cancer

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作  者:马涛[1] 张君莹[2] 吴建中[3] 唐金海[4] 

机构地区:[1]南京医科大学附属无锡市妇幼保健院乳腺外科,江苏无锡214002 [2]徐州医学院外科学系,江苏徐州221000 [3]南京医科大学附属江苏省肿瘤医院中心实验室,南京210000 [4]南京医科大学附属江苏省肿瘤医院乳腺外科,南京210000

出  处:《中南大学学报(医学版)》2014年第5期488-495,共8页Journal of Central South University :Medical Science

摘  要:目的:研究miR-342-3p对三阴性乳腺癌化疗敏感性的影响。方法:收集江苏省肿瘤医院乳腺外科2011年1月至2013年8月行术前化疗的三阴性乳腺癌病人32例,其中完全缓解(CR)5例,部分缓解(PR)27例,检测肿瘤组织中miR-342-3p的表达情况;应用脂质体转染方法,三阴性乳腺癌细胞株MDA-MB-231转染has-miR-342-3p模拟物(mimic)和miR-342-3p抑制物(inhibitor),设立阴性对照(mim-NC)组和(inhi-NC)组。mimic组,mim-NC组,inhibitor组和inhi-NC组细胞株,分别加入浓度为2μmol/L的紫杉醇,顺铂及4μmol/L的阿霉素进行培养;应用CCK8法和流式细胞仪检测48 h后肿瘤细胞增殖率和凋亡的变化。结果:miRNA-342-3p在术前化疗后达到CR的三阴性乳腺癌组织中的表达明显高于PR的三阴性乳腺癌组织(P<0.05)。Mimic组细胞株与紫杉醇,顺铂作用48 h后肿瘤细胞增殖率低于mim-NC组,凋亡率高于mim-NC组,差异有统计学意义(P<0.05)。Inhibitor组细胞株与紫杉醇,顺铂作用48 h后,肿瘤细胞增殖率高于inhiNC组,凋亡率低于inhi-NC组,差异有统计学意义(P<0.05)。但与阿霉素作用后细胞增殖率和凋亡率在mimic组与mimNC组,inhibitor组与inhi-NC组,差异无统计学意义(P>0.05)。结论:miR-342-3p高表达的三阴性乳腺癌对化疗药物反应更敏感,miR-342-3p能调控乳腺癌细胞株MDA-MB-231对紫杉醇和顺铂的化疗敏感性,但miR-342-3p不影响MDAMB-231细胞株对阿霉素的化疗敏感性。Objective: To study the effect of miR-342-3p on the chemotherapy sensitivity in triple-negative breast cancer(TNBC). Methods: Tissue samples were collected from January 2011 to August 2013 samples in Jiangsu Cancer Hospital from a total of 32 triple-negative breast cancer patients with preoperative chemotherapy, with 5 cases of complete response(CR) and 27 cases of partial response(PR). We detected miR-342-3p expression of TNBC with RT-PCR. We transfected has-miR-342-3p mimic and inhibitor into breast cancer cell lines MDA-MB-231 by lipofection transfection and set up negative control mim-NC and inhi-NC. Group of mimic, mim-NC, inhibitor and inhi-NC were cultivated with 2 μmol /L paclitaxel, cisplatin or 4 μmol/L doxorubicin for 48 h. h e cell growth rates were measured by CCK8 reagent kit, and the cell apoptosis rate by l ow cytometry. Results: h e expressions of miRNA-342-3p in TNBC tissue of CR were higher than those of PR. h e cell growth rates of mimic were lower and cell apoptosis rates were higher than those of minNC at er cultivating with paclitaxel or cisplatin for 48 h, with signii cant dif erence(P<0.05). h e cell growth rates of inhibitor were higher and cell apoptosis rates lower than those of inhi-NC at er cultivating with paclitaxel or cisplatin 48 h, with signii cant dif erence(P<0.05). h e cell growth and cell apoptosis rates of mimic and inhibitor had no dif erence with those of mim-NC and inhiNC at er cultivating with doxorubicin 48 h(P>0.05). Conclusion: TNBC with high expression of miR-342-3p are more sensitive to chemotherapy. miRNA-342-3p may regulate the sensitivity of breast cancer cell line MDA-MB-231 to chemotherapy drugs paclitaxel and cisplatin, but can not affect the chemotherapy sensitivity of doxorubicin.

关 键 词:三阴性乳腺癌 miR-342-3p 化疗敏感性 增殖 凋亡 

分 类 号:R737.9[医药卫生—肿瘤]

 

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