中药紫龙金与HMBA对人胃癌不同周期细胞基因表达调控的共性研究  

Common character of Zilongjin and HMBA as for gene expression regulation in gastric cancer cell cycle

作　　者：李宝园 梁云燕[1] 王代树[1] 

机构地区：[1]北京市肿瘤防治研究所细胞室

出　　处：《山西医科大学学报》2004年第4期321-323,共3页Journal of Shanxi Medical University

基　　金：国家自然科学基金资助项目 ( 3 9480 0 2 6);北京市科委重大项目 ( 85 2 60 3 0 0 )

摘　　要：目的　探讨中药紫龙金与环六亚甲基二己酰胺 (HMBA)对人胃癌MGc80 3不同周期细胞癌基因c ras、c myc、抑癌基因Rb、p5 3、p2 1以及蛋白激酶PKC a基因表达的影响。方法　采用Northernblot印迹法检测基因表达情况。结果　MGc80 3不同周期时相细胞用中药紫龙金和HMBA分别处理后 ,癌基因ras、myc表达下降 ,多数抑制率达到 5 0 %以上 ;促进细胞增殖的蛋白激酶PKC基因表达基本与癌基因表达抑制相似。两类药物对抑癌基因的影响则有显著差异 ,HMBA处理后Rb和 p2 1在G1期细胞的表达下降抑制率达 39.5 %和 33.3% ,Rb在S期也抑制 3.0 % ;而中药紫龙金对Rb和 p2 1在各个周期细胞均是促进表达 ,多数时相达 12 5 .0 %～ 2 33.4 %。结论　中药紫龙金与HMBA对癌基因和抑癌基因表达的调节作用基本相似 ,但紫龙金作用优于HMBA ,两类药物对MGc80 3细胞的增殖抑制和促分化作用的分子机制和两类药物对细胞周期内癌基因与抑癌基因调节相关。Objective To investigate the effects of the Chinese drug Zilongjin and hexamethylene bisacetamid( HMBA) on expression of oncogenes(c-ras,c-myc),and tumorsuppressor genes(Rb,p53,p21)of MGc803 cell in cell cycle. Methods RNA Northern blot was used to survey the levels of gene expression of MGc803 differentphase cells treated with Zilongjin and HMBA respectively. Results In different phases of MGc803 cell expression of oncogenes c-ras and c-myc were inhibited by 50.5%. The protain kinase subspecies PKC-a gene was similar to the expression inhibition of oncogenes, except for effect of Zilongjin on the G 1 phase in cell cycle. The effects of Zilongjin differed greatly from HMBA in expression of tumor suppressor genes. The expression of Rb and P21 in cells treated withHMBA were inhibited by 39.5% and 33.3%. The level ofRbgene expression deceased too by 3.0%in phase. The expression of Rb and p21 genes increased in all cell cycle after being treated with Zilongjin. But the expression of p53 gene increased obviously by 125.0% and 233.4% in majority phases of MGc803 cells which was similar to the HMBA. Conclusions Effects of Zilongjin on theregulation of oncogenes and tumor suppressor genes are similar to HMBA, but theeffects of Zilongjin are better than that of HMBA. Molecular mechanism of the two drugs on the proliferation inhibition differentiation of MGc803 cells is related to regulation of the oncogenes and tumor suppressor genes in cell cycle of MGc803 cells.

关 键 词：中成药 紫龙金 环六亚甲基二己酰胺 细胞周期 癌基因 基因 肿瘤抑制 

分 类 号：R73[医药卫生—肿瘤]