高效毛细管电泳法分离测定5种水溶性维生素  

Separation and Determination of Five Kinds of Water-soluble Vitamins by High Performance Capillary Electrophoresis

作　　者：柯月娇 黄桂华[1] 张苏娜[1] 李琦[1] 

机构地区：[1]福建中医药大学药学院,福建福州350122

出　　处：《福建中医药大学学报》2014年第1期27-29,共3页Journal of Fujian College of Traditional Chinese Medicine

基　　金：国家自然科学基金资助课题(81001634);福建中医药大学校管课题(XB2011008)

摘　　要：目的建立高效毛细管电泳同时分离测定5种水溶性维生素的方法。方法采用高效毛细管电泳法分离测定复合维生素B片和脉动饮料中的维生素,研究缓冲溶液体系、电压、温度、进样时间对测定结果的影响。结果 5种水溶性维生素在6 min内获得基线分离。结论高效毛细管电泳法对水溶性维生素的分析具有简便、快速的特点。Objective To establish a high performance capillary electrophoresis(HPCE) method for the simultaneous separation and determination of five kinds of water-soluble vitamins. Methods The vitamins in Compound Vitamin B Tablets and Mizone were separated and determined by HPCE method, and the effects of buffer solution system, separation voltage, column temperature and sample injection time on determination results were studied. Results The base line separation of five kinds of water-soluble vitamins was obtained within 6 minutes. Conclusion The method is simple and rapid, which can be used for analysis of water-soluble vitamins.

关 键 词：高效毛细管电泳 水溶性维生素 维生素B片 

分 类 号：R284.2[医药卫生—中药学]