大黄鱼(Pseudosciaena crocea)内脏白点病病原分离鉴定及致病性研究  

ISOLATION, IDENTIFICATION AND VIRULENCE OF THE PATHOGEN OF WHITE-SPOTS DISEASE IN INTERNAL ORGANS OF PSEUDOSCIAENA CROCEA

作　　者：胡娇[1] 张飞[1] 徐晓津 苏永全[2] 覃映雪 马英 张艺 韩坤煌 鄢庆枇 

机构地区：[1]集美大学水产学院农业部东海海水健康养殖重点实验室,厦门361021 [2]厦门大学海洋与地球学院,厦门361005 [3]宁德市水产技术推广站,宁德352101 [4]宁德市富发水产有限公司,宁德352103

出　　处：《海洋与湖沼》2014年第2期409-417,共9页Oceanologia Et Limnologia Sinica

基　　金：公益性行业(农业)科研专项;200903029号;国家自然科学基金项目;31272699号;41176115号

摘　　要：于2013年4月从宁德患内脏白点病大黄鱼(Pseudosciaena crocea)中分离得到两株优势菌NZBD9和NZBD11,这两株菌在16—19°C条件下回归感染能引起大黄鱼内脏白点病,而在7—10°C和24—27°C条件下同样的人工感染不能致病,从而证实这两株菌为大黄鱼内脏白点病的病原菌。经16S rDNA基因的测序和时间飞行质谱微生物鉴定仪分析,NZBD9和NZBD11同为变形假单胞菌。药敏性实验结果显示NZBD9对庆大霉素、诺氟沙星和四环素等7种药物高度敏感。组织病理学观察结果显示病鱼的肝脏、脾脏、头肾等组织中均出现明显病症,如变性和坏死。Two dominant strains(NZBD9 and NZBD11) were isolated from internal organs of Pseudosciaena crocea suffered from white-spots disease in April 2013. Both strains could cause the disease artificially in the internal organs in 16—19℃ but at 7—10℃ and 24—27℃. The results indicate that the two strains were the causative agent of the disease in farmed P. crocea. Both were identified as Pseudomonas plecoglossicida by time-of-flight mass spectrometer in 16S rDNA sequences. Experiments in susceptibility showed that NZBD9 is highly sensitive to 7 drugs including gentamicin, norfloxacin, and tetracycline. Histopathological observation showed that affected fish had clear symptoms of degeneration and necrosis in liver, kidney and spleen.

关 键 词：大黄鱼 变形假单胞菌 内脏白点病 致病性 

分 类 号：S943[农业科学—水产养殖]