瘦素对牛体外胚胎早期发育阻滞的克服  

Effects of Leptin on in vitro Early Embryonic Development Diapause

作　　者：阎芃[1] 倪和民[1] 刘云海[1] 郭勇[1] 梁琳[1] 郑雪莹[1] 

机构地区：[1]北京农学院动物科学技术学院,北京102206

出　　处：《畜牧兽医学报》2014年第5期763-768,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：2011年度国家自然基金面上项目(31072185);2013年度北京市教委北京市属高等学校创新团队建设与教师职业发展计划项目(PXM2013_014207_000067);2013年度国家自然基金面上项目(31272526)

摘　　要：旨在研究瘦素(Leptin)对于牛早期体外胚胎发育阻滞的影响。本研究采用:(1)卵母细胞受精后在不含血清情况下加入不同浓度的Leptin(0、1、10、100ng·L-1 Leptin并以血清组为对照),观察胚胎的发育能力;(2)用RT-PCR方法对正常发育胚胎及阻滞胚胎的Leptin mRNA表达量进行检测;(3)用RT-PCR方法对添加外源性Leptin胚胎的L-R、STAT3mRNA表达量进行检测。结果表明:(1)在卵母细胞受精后加入10或100ng·L-1Leptin胚胎的卵裂率及囊胚率显著高于对照组(P<0.05);(2)正常发育胚胎的Leptin mRNA的表达量显著高于阻滞组(P<0.05);(3)添加外源性Leptin胚胎L-R、STAT3mRNA表达量显著高于对照组(P<0.05)。综上表明:Leptin在牛早期胚胎发育过程中起到重要作用,Leptin和L-R表达量降低预示着胚胎发育停止,并通过JAK/STAT3途径发挥作用。The experiment was conducted to study Leptin on in vitro early embryonic development diapause.The different concentration of Leptin 0,1,10,and 100ng·L-1 were added to IVF early embryo medium,using 1% FCS as control group.The bovine in vitro embryos development with different concentration of Leptin were observed.RT-PCR was used to detect the expression of Leptin mRNA of normal and arrested embryo and the effect of Leptin on L-Rand STAT3mRNA expression.The results showed that:(1)Embryo cleavage rate and blastocyst rate of addition 10, 100ng·L-1 Leptin is significantly higher than control group(P<0.05);(2)The expression of Leptin mRNA in normal embryo was significantly higher than that of arrested embryo(P< 0.05);(3)The expression of L-R,STAT3mRNA of in vitro early embryo with exogenous Leptin was significantly higher than that of the control one(P<0.05).In conclusion,Leptin is very important to bovine in vitro early embryonic development,less expression of Leptin and L-Ris mark to embryonic development diapause,and these process play a role in JAK/STAT3pathway.

关 键 词：LEPTIN 早期体外胚胎 发育阻滞 

分 类 号：S823[农业科学—畜牧学]