水通道蛋白1及其mRNA在胸膜恶性肿瘤进展中的作用  

Effect of Aquaporin 1 and Its mRNA in Mice with Malignant Pleural Tumor

作　　者：张靖轩[1] 张伟[2] 周华荣[3] 谢慧文[2] 董洪珍[2] 肖曼[2] 

机构地区：[1]广州市第一人民医院中心ICU,广州510000 [2]广州中医药大学第一附属医院呼吸内科 [3]中山大学孙逸仙纪念医院南院区急诊科

出　　处：《肿瘤防治研究》2014年第4期297-300,共4页Cancer Research on Prevention and Treatment

基　　金：广东省自然科学基金资助项目(S2012010010563)

摘　　要：目的探讨胸膜恶性肿瘤的进展对水通道蛋白1(aquaporin-1,AQP1)及其mRNA的影响。方法通过胸膜腔注射法建立胸膜恶性肿瘤的动物模型,按肿瘤种植时间分组(T6,T8,T10),记录各组胸水量,采用免疫组织化学法、Real-Time PCR的方法测定AQP1及AQP1-mRNA水平。结果肿瘤小鼠胸水量随时间而增长;壁层胸膜AQP1及其mRNA水平随肿瘤进展而增高,且与胸水量呈正相关。结论在胸膜恶性肿瘤的进展中,胸膜壁层AQP1及其mRNA水平升高,胸水量与AQP1、AQP1-mRNA水平呈正相关,进而提示AQP1参与了胸膜恶性肿瘤的构建和进展。Objective To reveal the change of aquaporin 1(AQP1) in malignant pleural tumor and their relationship. Methods Establish animal model of C57BL/6J mice with malignant pleural tumor, the tumor group was divided into three groups(T6,T8,T10). Based on the sixth, eighth, tenth day of injection, these mice were sacrifi ced and record the volume of the malignant pleural effusion, and determine the express of AQP1 and its mRNA in partial pleura using immunohistochemistry and Real-time PCR. Results 21 mice in MPE team appeared malignant pleural effusion in the right pleural cavity, MPE amount,level of VEGF and AQP1of the 3 groups was gradually increased. There was positive correlation between the level of AQP1 and VEGF in T6, T8, T10 group and the amount of MPE. Conclusion AQP1 participates in the formation of malignant pleural tumor, its level and the amount of pleural fl uid are positive correlation. Inhibiting the activition of AQP1 may be new ways to treat the malignant pleural tumor.

关 键 词：胸膜肿瘤 恶性 水通道蛋白1 

分 类 号：R734.3[医药卫生—肿瘤]