天麻素对化疗痛模型大鼠脊髓IL-1β和TNFα表达的影响  

Effects of Gastrodin on Expression of IL-1β and TNFα of Spinal Cord in Chemotherapy-induced Neuropathic Pain

作　　者：张金芝[1] 江永祥 郑卫红[1] 宫登辉[1] 罗妮[1] 

机构地区：[1]三峡大学医学院国家中药药理科研三级实验室,湖北宜昌443002 [2]宜昌市公安局西陵分局司法鉴定中心

出　　处：《肿瘤防治研究》2014年第4期301-304,共4页Cancer Research on Prevention and Treatment

基　　金：湖北省自然科学基金资助项目(2013CFC066);湖北省教育厅科技研究重点资助项目(D20101208);三峡大学研究生科研创新基金资助项目(2011CX060);宜昌市科学研究与开发资助项目(A13301-57)

摘　　要：目的观察天麻素对长春新碱致大鼠神经病理性疼痛的治疗作用,观察大鼠脊髓腰段IL-1β和TNFα表达的变化,探讨其在脊髓水平的作用机制。方法用长春新碱隔日腹腔注射制作大鼠化疗痛模型,模型成功制备后腹腔注射天麻素治疗,用电子Von Frey测痛仪测定大鼠机械性痛阈,热辐射仪测定大鼠热刺激痛阈值;采用ELISA方法检测脊髓腰段IL-1β和TNFα表达情况。结果实验第8天化疗痛大鼠模型成功建立,用不同剂量天麻素治疗模型大鼠1周后,治疗组大鼠的机械和热刺激痛阈值与模型组比较均有不同程度的提高(P<0.01,P<0.05);与模型组比较,治疗组大鼠的IL-1β和TNFα的表达明显降低(P<0.01)。结论天麻素可减轻长春新碱诱导的大鼠化疗痛反应,其机制可能与抑制化疗痛大鼠脊髓IL-1β和TNFα的表达有关。Objective To observe the treatment action of gastrodin on chemotherapy-induced neuropathic pain in rats, and the changes of IL-1 β and TNFα expression in spinal cord, and to study the mechanism in the spinal cord level. Methods Vincristine was administered intravenously on alternate days to establish the model of chemotherapy-induced neuropathic pain, after the model was established successfully, gastrodin was administered intravenously, mechanical allodynia was measured using electronic Von Frey, heat hyperalgesia was measured by electronic heatingplaten instrument; The expression of IL-1β and TNFα was detected using ELISA technique. Results Vincristine-induced neuropathic pain model was established successfully on the eighth day, after one week treatment with different doses of gastrodin, mechanical pain and heat pain threshold were differently improved in different dose treatment groups compared with the model group(P<0.01, P<0.05); In model group, the expression of IL-1β and TNFα was enhanced signifi cantly in spinal cord(P<0.01).Conclusion Gastrodin could relieve the response to vincristine-induced pain. The mechanism of action may be related to inhibiting the expression of IL-1β and TNFα in spinal cord level.

关 键 词：天麻素 化疗痛 白细胞介素-1β 肿瘤坏死因子α 长春新碱 

分 类 号：R730.53[医药卫生—肿瘤]