洛铂对骨肉瘤MG-63细胞增殖、凋亡的影响及其机制  

Effects of Lobaplatin on Proliferation and Apoptosis of Osteosarcoma MG-63 Cells and Its Mechanism

作　　者：冯和林[1] 郑丽华[2] 赵亚恒[2] 李增怀[1] 褚彦青[1] 冯建刚[1] 吴宏增[1] 单保恩[3] 

机构地区：[1]河北医科大学第四医院骨科,石家庄050011 [2]河北医科大学研究生学院 [3]河北医科大学第四医院科研中心河北省肿瘤研究所

出　　处：《肿瘤防治研究》2014年第5期358-362,共5页Cancer Research on Prevention and Treatment

基　　金：河北省科技厅支撑课题资助项目(11276156)

摘　　要：目的探讨洛铂对骨肉瘤MG-63细胞增殖与凋亡的作用及其机制。方法取对数生长期的MG-63细胞进行实验,分为对照组和实验组(加入不同浓度洛铂:2、4和8μg/ml)。采用噻唑盐(MTT)比色法、流式细胞技术(FCM)检测洛铂对MG-63细胞的形态改变、生长及增殖抑制、细胞周期阻滞和细胞凋亡诱导等作用,并绘制不同浓度时MG-63细胞的生长曲线,应用Western blot的方法分析洛铂对MG-63细胞的Bcl-2蛋白表达的影响。结果洛铂能够抑制MG-63细胞的生长、增殖并诱导其凋亡,并呈浓度和时间依赖效应。Western blot检测结果显示洛铂可使MG-63细胞Bcl-2蛋白表达下降。结论洛铂能显著抑制MG-63细胞增殖,诱导细胞凋亡和细胞周期变化,可能与调节MG-63细胞Bcl-2蛋白的表达有关。Objective To study the effects of lobaplatin on the proliferation and apoptosis of osteosarcoma MG-63 cells and the mechanism of its function. Methods MG-63 cells in log phase were divided into control group and experimental groups. Experimental groups were treated with different concentrations of lobaplatin(2, 4 and 8μg/ml). MTT colorimetry and fl owcytometry were used to detect the effects of lobaplatin on cellular morphology change, cell growth, cell cycle, and the induction of apoptosis in MG-63 cells. Cell growth curves were studied at different concentrations of lobaplatin. Western blot was used to detect the effects of lobaplatin on the expression of Bcl-2 in MG-63 cells. Results Lobaplatin could inhibit the growth or proliferation and increase the apoptosis of osteosarcoma cell line MG-63 in a dose- and time-dependent manner. Bcl-2 protein level was decreased after lobaplatin treatment. Conclusion The effects of lobaplatin on the proliferation, the apoptosis and changing the cell cycle change of MG-63 cells might be related to its inhibition on Bcl-2 protein expression.

关 键 词：洛铂 骨肉瘤 MG-63细胞 凋亡 BCL-2 

分 类 号：R738.1[医药卫生—肿瘤]