靶向HER2基因shRNA对小鼠Lewis细胞化疗敏感度的影响  

Effects of HER2 shRNA on Chemotherapy Sensitivity of Mouse Lewis Cells

作　　者：曹新梅[1] 张岱权[2] 夏纪毅[3] 王栩[1] 高燕[1] 熊伟[4] 

机构地区：[1]泸州医学院免疫学教研室,四川泸州646000 [2]泸州医学院附属医院中医科 [3]泸州医学院药物与功能性食品研究中心 [4]泸州医学院公共卫生系

出　　处：《肿瘤防治研究》2014年第5期366-368,共3页Cancer Research on Prevention and Treatment

基　　金：四川省卫生厅基金资助项目(100236)

摘　　要：目的探讨靶向HER2基因shRNA对小鼠肺腺癌Lewis细胞化疗敏感度的影响。方法应用Lipofectamine 2000将pGPU6/RFP/Neo-erbB-2、pGPU6/RFP/Neo-shNC质粒表达载体快速转染小鼠肺腺癌Lewis细胞;应用RT-PCR、Western blot检测各组HER2 mRNA、蛋白的表达。应用流式细胞术检测未转染对照组、pGPU6/RFP/Neo-shNC组、pGPU6/RFP/Neo-erbB-2组、卡铂组、pGPU6/RFP/Neo-shNC+卡铂组、pGPU6/RFP/Neo-erbB-2+卡铂组的细胞凋亡率。结果 pGPU6/RFP/Neo-erbB-2组HER2 mRNA和蛋白的水平均低于pGPU6/RFP/Neo-shNC组和未转染对照组。pGPU6/RFP/Neo-erbB-2+卡铂组细胞的凋亡率高于其余各组(P均<0.01)。结论 HER2 shRNA能增强小鼠Lewis细胞对卡铂的敏感度。Objective To investigate the effects of HER2 shRNA on the chemotherapy sensitivity of Mice Lewis cells.Methods pGPU6/RFP/Neo-erbB-2 and pGPU6/RFP/Neo-shNC plasmids were synthesized and transfected into mice Lewis cells by Lipofectamine 2000.The levels of HER2 mRNA and protein were tested by RT-PCR and Western blot, respectively.The apoptotic rates of cells in non-transfected group, pGPU6/RFP/ Neo-shNC group,pGPU6/RFP/Neo-erbB-2 group,Carboplatin group, pGPU6/RFP/Neo-shNC plus Carboplatin group and pGPU6/RFP/Neo-erbB-2 plus Carboplatin group were tested by FCM.Results The levels of HER2 mRNA and protein in pGPU6/RFP/Neo-erbB-2 group were lower than those in pGPU6/RFP/Neo-shNC group and non-transfected group.The apoptotic rate of cells in pGPU6/RFP/Neo-erbB-2 plus carboplatin group was higher than those in other groups(all P<0.01).Conclusion HER2 shRNA could increase the sensitivity of mouse Lewis cells to carboplatin.

关 键 词：HER2 SHRNA Lewis细胞 化疗敏感度 

分 类 号：R734.2[医药卫生—肿瘤]