肝癌组织中FMNL-2和MMP-2的表达及临床病理分析  

Expression of FMNL-2 and MMP-2 in Primary Hepatocellular Carcinoma and Its Clinicopathological Characteristics

作　　者：罗佳[1] 章敬成[1] 刘繁荣[1,2] 吴冬梅[1] 廖首生[1] 杨宇晴[1] 

机构地区：[1]南昌大学第二附属医院病理科,南昌330006 [2]南昌大学医学院病理教研室

出　　处：《肿瘤防治研究》2014年第5期460-463,共4页Cancer Research on Prevention and Treatment

基　　金：江西省教育厅科技计划资助项目(20100611);江西省科技支撑计划重点项目(2010BSA13100)

摘　　要：目的探讨肝癌及癌旁组织中FMNL-2和MMP-2的蛋白表达及与临床病理特征的关系。方法采用免疫组织化学MaxvisionTM法检测100例肝癌及癌旁组织中FMNL-2和MMP-2的蛋白表达情况,应用图文分析软件Image-Pro Plus6.0测定FMNL-2和MMP-2的蛋白累积吸光度值(IOD),采用SPSS19.0软件统计分析。结果肝癌及癌旁组织中FMNL-2蛋白IOD值分别为(90 748.36±46 276.91)和(39 075.46±24 763.12),MMP-2蛋白IOD值分别为(87 969.46±27 131.77)和(59 517.72±20 218.23),两组差异均有统计学意义(P<0.05);FMNL-2在伴与不伴肝硬化组中IOD值分别为(47 471.96±40 273.54)和(72 123.41±45 517.93)(P<0.05);MMP-2在HBsAg阳性或阴性组中IOD值分别为(77 668.34±27 682.79)和(64 585.83±26 173.62)(P<0.05),在有无转移组中IOD值分别为(63 854.73±21730.42)和(77 218.05±28 930.01)(P<0.05),差异有统计学意义;并且肝癌及癌旁组织中FMNL-2和MMP-2蛋白表达呈正相关(r=0.240,P=0.016)。结论在肝癌发生、发展过程中存在FMNL-2和MMP-2蛋白异常表达,联合监测两者蛋白表达可能会有助于肝癌的诊断。Objective To invesstigate the expression of FMNL-2 and MMP-2 in hepatocellular carcinoma(HCC) and the adjacent tissues, and to detect their relationship with the clinicopathological features. Methods The protein expression of FMNL-2 and MMP-2 were detected by immunohistochemistry MaxvisionTM method in the cancer tissues and adjacent tissues from 100 patients. Integrated optical density(IOD) of immunohistochemical image was estimated by Image-ProPlus 6.0 software. Statistical analysis was performed by SPSS19.0 software. Results IOD of FMNL-2 between cancer tissues and adjacent tissues were(90 748.36±46 276.91)and(39075.46±24763.12);IOD of MMP-2 between cancer tissues and adjacent tissues were(87 969.46±27 131.77) and(59 517.72±20 218.23)(P<0.05); IOD of FMNL-2 between cirrhosis of liver tissue and non-cirrhosis liver tissue were(47 471.96±40 273.54) and(72 123.41 ±45 517.93), respectively(P<0.05); IOD of MMP-2 between HBsAg positive and negative group were(77 668.34±27 682.79) and(64 585.83±26173.62),respectively(P<0.05),IOD of MMP-2 between metastasis and without metastasis group were(63 854.73±21 730.42) and(77 218.05±28 930.01)(P<0.05),FMNL-2 expression was positively correlated to that of MMP-2 in cancer tissues and adjacent tissues(r=0.240,P=0.016). Conclusion FMNL-2 and MMP-2 proteins are abnormally expressed in hepatocellular carcinoma, indicating that detection of FMNL-2 and MMP-2 proteins may be helpful for diagnosis of hepatocellular carcinoma.

关 键 词：肝细胞癌 成蛋白相关蛋白2(FMNL-2) 基质金属蛋白酶-2 

分 类 号：R735.7[医药卫生—肿瘤]