氯化锂对白血病THP-1细胞生长的抑制作用及其对Wnt通路的影响  

Lithium Chloride Inhibits Proliferation of THP-1 Leukemia Cells and Activates Wnt Signaling Pathway

作　　者：许思苗[1] 方勇[2] 周密 赵雪娇 秦宇 张沛凌 原瑞凤 周剑峰 杨漾 

机构地区：[1]华中科技大学同济医学院附属同济医院血液科,武汉430030 [2]华中科技大学同济医学院附属同济医院妇产科,武汉430030

出　　处：《肿瘤防治研究》2014年第6期568-572,共5页Cancer Research on Prevention and Treatment

基　　金：国家973科学基金资助项目(2009CB521806)

摘　　要：目的研究氯化锂(LiCl)在体外对白血病细胞THP-1增殖、凋亡、周期以及Wnt通路的影响。方法氯化锂不同浓度不同时间作用THP-1细胞后,用MTT法检测细胞增殖,流式细胞术检测细胞凋亡和周期分布,Western blot检测细胞内Wnt通路的变化。结果氯化锂能抑制白血病THP-1细胞的增殖,诱导细胞凋亡,使细胞周期阻滞于G2/M期,且均呈浓度和时间依赖性。氯化锂不同浓度不同时间作用THP-1细胞后,t-GSK3β、cyclinD1表达不变,p-GSK3β、β-catenin表达升高,短时间低浓度作用后c-myc表达升高,但随作用时间延长和浓度增加,c-myc表达降低。结论氯化锂对THP-1细胞有显著的增殖抑制、凋亡诱导、细胞G2/M期阻滞作用,并激活Wnt通路,影响Wnt通路下游蛋白c-myc、cyclinD1的表达。Objective To explore the effects of Lithium Chloride(LiCl) on proliferation, apoptosis and cell cycle of leukemia cells THP-1, and to investigate its effect on Wnt signaling pathway. Methods THP-1 cells were treated with LiCl at different concentrations and time. The proliferation inhibitory rates of THP-1 cells were assayed by MTT. The apoptosis rates and the cell cycle distribution were detected by flow cytometry. The expressions of Wnt signaling pathway proteins were analyzed by Western blot. Results LiCl could inhibit the proliferation and induce the apoptosis of THP-1 cells. LiCl mainly arrested THP-1 cells in the G2/M phase in a time- and dose-dependent manner. Western blot results suggested that the expressions of t-GSK3β and cyclinD1 had no obvious change. The expressions of p-GSK3β and β-catenin were increased. The expression of c-myc protein was increased with short-time and low-concentration treatment, while decreased with long-time and high-concentration treatment. Conclusion LiCl could inhibit proliferation, induce apoptosis and arrest cell cycle of THP-1 cells. In addition, it could activate Wnt signaling pathway, and influence the expressions of c-myc and cyclinD1 proteins.

关 键 词：氯化锂 白血病 THP-1细胞系 增殖 凋亡 细胞周期 糖原合成酶激酶 WNT信号通路 

分 类 号：R73[医药卫生—肿瘤]