siRNA靶向沉默Smad7对PC12细胞氧糖剥夺敏感性的影响  

Effects of RNA interference targeting Smad7 on oxygen-glucose deprivation sensitivity of PC12 cell

作　　者：王一鸣[1] 程也[1] 付丹阳[1] 王勇涛[1] 王姣琦[2] 梅春丽[2] 莽靖[2] 徐忠信[2] 

机构地区：[1]吉林大学白求恩医学部临床医学院,吉林长春130021 [2]吉林大学中日联谊医院神经内科

出　　处：《中国老年学杂志》2014年第6期1535-1537,共3页Chinese Journal of Gerontology

基　　金：国家自然基金面上项目(81371298);吉林省自然基金项目(201015181);吉林省科技发展计划国际合作项目(20120723);吉林省产业技术研究与开发项目(2013C030-4);吉林大学大学生创新基金项目(2013A71245)

摘　　要：目的探讨siRNA靶向沉默Smad7基因对PC12细胞氧糖剥夺(OGD)损伤的影响及其相关机制。方法体外建立PC12细胞OGD模型,利用RNA干扰技术,沉默Smad7基因表达,实时RT-PCR及Western印迹检测Smad7基因的表达,Hoechst33342染色检测细胞凋亡情况,实时RT-PCR检测Smad3基因mRNA的表达变化。结果 siRNA转染下调Smad7基因mRNA及蛋白的表达,靶向沉默Smad7联合OGD 16 h组细胞凋亡较OGD 16 h组明显减少;Smad7低表达的PC12细胞内Smad3 mRNA的表达明显增加。结论 Smad7基因沉默降低PC12细胞对OGD损伤的敏感性,提高Smad3基因在转录水平上的表达。Objective To explore the protective effect of target silencing Smad7 gene through siRNA in PC12 cell treated by oxygenglucose deprivation( OGD) and the related mechanism. Methods PC12 cell was exposed to OGD treatment in vitro. RNA interference technology was used to reduce the expression of Smad7 gene,which was detected by real time RT-PCR and Western blot. The apoptosis of cells was detected by Hoechst33342 staining. Real time RT-PCR was used to test the expression of Smad3 mRNA. Results The mRNA expression of Smad7 was significantly reduced after siRNA transfection targeting to Smad7. Hoechst33342 showed the apoptosis of cells in OGD16h combined with Smad7 gene silencing group was decreased compared to that of OGD16h group. The expression of Smad3 mRNA was increased in Smad7 gene silencing group. Conclusions Smad7 gene silencing can reduce the sensitivity of PC12 cell to OGD damage and promote the transcriptional expression of Smad3.

关 键 词：ActA/Smads SMAD3 SMAD7 SIRNA 氧糖剥夺 

分 类 号：R363[医药卫生—病理学]