氢饱和盐水对阿尔茨海默病模型大鼠氧化应激损伤的影响  

Hydrogen-rich saline improving learning and memory abilities of a rat model of Alzheimer's disease

作　　者：李健[1] 刘春娜[2] 刘新宇[1] 李熙东[1] 刘媛媛[1] 

机构地区：[1]辽宁医学院附属第一医院神经内科,辽宁锦州121001 [2]辽宁医学院药理教研室

出　　处：《中国老年学杂志》2014年第10期2746-2749,共4页Chinese Journal of Gerontology

基　　金：国家自然科学基金(No.81201037);辽宁省博士启动基金计划(No.20131068);辽宁医学院博士启动项目(No.Y2012B015)

摘　　要：目的探索氢饱和盐水对阿尔茨海默病(AD)模型大鼠氧化应激的影响。方法选用280~320 g Sprague-Dawley(SD)大鼠,随机分为模型组、假手术组、氢饱和盐水治疗组;利用立体定向技术侧脑室注射Aβ1~42建立AD模型大鼠,腹腔注射氢饱和盐水。给药14 d后采用生物化学法检测各组大鼠的脑海马组织匀浆的MDA含量,利用免疫组织化学法检测各组大鼠脑海马组织氧化应激标志物HNE及8-OH-d G蛋白的表达。结果模型组大鼠脑组织MDA含量为(6.65±1.07)nmol/mgprotein,与假手术组相比显著升高(P=0.000),而氢饱和盐水治疗组大鼠脑组织的MDA含量较模型组大鼠显著降低(P=0.016)。模型组大鼠海马齿状回显现较多的HNE阳性细胞,假手术组大鼠鲜见HNE阳性细胞,而氢饱和盐水治疗组大鼠海马齿状回HNE阳性细胞较模型组大鼠显著减少(P=0.011)。海马齿状回呈现棕黄色8-OH-d G染色阳性细胞,与假手术组大鼠相比,模型组大鼠海马齿状回出现明显增多的8-OH-d G染色阳性细胞(P=0.000),而经氢饱和盐水治疗后的模型大鼠海马齿状回8-OH-d G染色阳性细胞明显减少(P=0.000)。顶叶皮层呈现棕黄色8-OH-d G染色阳性细胞,与假手术组大鼠相比,模型组大鼠顶叶皮层出现明显增多的8-OH-d G染色阳性细胞(P=0.000),而经氢饱和盐水治疗后的模型大鼠顶叶皮层8-OH-d G染色阳性细胞明显减少(P=0.000)。结论氢饱和盐水可显著减少Aβ诱导的氧化应激损伤蛋白及核酸标志物的表达。Objective To explore the effect of hydrogen-rich improving learning and memory abilities of a rat model of Alzheimer's disease(AD).Methods S-D male rats (n=84, 280~330 g) were divided into sham-operated, Aβ1~42injected and Aβ1~42 plus hydrogen-rich saline-treated groups.Hydrogen-rich saline (5 ml/kg, i.p., daily) was injected for 14 days after intracerebroventricular injection of Aβ1~42.Morris water Maze and open field task were used to assess the memory dysfunction and motor dysfunction , respectively.LTP was used to detect the electrophysiology changes.Results In training trials, the escape latency time on the last training day (the fifth day) was (10.64±8.62) s in sham group, (30.10±20.93) s in Aβ1~42 plus physiological saline group, and (18.14±16.16) s in Aβ1~42 plus hydro-gen-rich saline group.In probe trials, animals in hydrogen-rich saline group spent more time on the right quadrant for searching the platform when compared with that of Aβ1~42 plus physiological saline group ( P=0.026 ).No alterations of the swimming speed ( F=1.73;P=0.18 ) in the Morris water Maze , or the total squares crossed ( F=0.81;P=0.45 ) and rearing behavior ( F=0.62;P=0.54 ) in the open field are-na were observed.In sham group , 200 Hz conditioning stimulation induced a robust and stable LTP of the EPSP amplitude compared to pre -HFS baseline〔(177±5)%of baseline at 1 h post-HFS〕, and the LTP was produced in the Aβ1~42(2.2 nmol) group〔(127±6)%〕following HFS( P=0.000 ) , and which was attenuated significantly in hydrogen-rich saline group〔( 148 ±8 )%〕( P=0.000 ) .Conclusions Hydro-gen-rich saline could improve amyloid β( Aβ) induced learning and memory deficits of AD model rats .

关 键 词：阿尔茨海默病 氢饱和盐水 氧化应激 4-羟基壬烯醛 8-羟基脱氧鸟苷 

分 类 号：R749.16[医药卫生—神经病学与精神病学]