不同时间脑缺血/再灌注损伤小鼠大脑Bcl-2和Caspase-3表达的变化  

Sutdy on the experssion and significance of Bcl-2 and Caspase-3 in different time of brain mice ischemia /reperfusion injury mice

作　　者：崔芳芹[1] 杨卫东[2] 陈前芬[1] 赵云霞[1] 

机构地区：[1]蚌埠医学院病理生理学教研室,安徽蚌埠233030 [2]蚌埠医学院机能实验中心

出　　处：《中国老年学杂志》2014年第10期2765-2767,共3页Chinese Journal of Gerontology

基　　金：安徽省教育厅自然科学研究项目(KJ2013Z209);蚌埠医学院院课题基金(BY1004)

摘　　要：目的观察不同缺血时间所引起的再灌注损伤小鼠脑神经细胞Bcl-2、Caspase-3蛋白表达的变化。方法健康昆明种小鼠50只,随机均分为五组,假手术组:仅行手术,不实施脑缺血;I/RⅠ组:缺血30 min,再灌注1 h;I/RⅡ组:缺血60 min,再灌注1 h;I/RⅢ组:缺血90 min,再灌注1 h;I/RⅣ组:缺血120 min,再灌注1 h;取小鼠脑组织,进行免疫组织化学染色(SP法)和计算机图像分析。结果在一定缺血时间范围内,Bcl-2随缺血时间的延长而表达增加,其中I/RⅡ组Bcl-2的平均光密度(MOD)值分别与假手术组和I/RⅠ组比较,均有显著性差异(P<0.05);但缺血时间进一步延长,I/RⅢ组和I/RⅣ组Bcl-2表达逐渐减少,分别与I/RⅡ组Bcl-2的MOD值比较,均有显著性差异(P<0.05)。Caspase-3随着缺血时间的延长而表达不断增加,I/RⅠ组、I/RⅡ组和I/RⅢ组Caspase-3的MOD值分别与假手术组比较,其差异均有显著性(P<0.05);但缺血时间过久,I/RⅣ组Caspase-3表达减弱,与I/RⅢ组Caspase-3的MOD值比较,有显著性差异(P<0.05)。结论在缺血早期受损神经元内,Bcl-2和Caspase-3表达均上调且Caspase-3占优势,但随着缺血时间的进一步延长,Bcl-2表达显著减少,为临床上早期使用Caspase抑制剂和Bcl-2激动剂治疗急性脑缺血性疾病提供实验室依据。Objective To observe the expression and significance of Bcl-2 and Caspase-3 in brain neurons cells of the different is-chemia reperfusion injury of mice and for the treatment and prognosis of acute cerebral ischemic diseases clinically provide laboratory basis . Methods 50 healthy Kunming mice were randomly divided into the following 5 groups:control group:only with surgery , not implement cer-ebral ischemia;ischemia-reperfusion groupⅠ( I/RⅠgroup ) , 30 min of ischemia and reperfusion 1 h; ischemia-reperfusion Ⅱ groups:60 min of ischemia and reperfusion 1 h group ( I/RⅡgroup);ischemia-reperfusion Ⅲgroup:90 min of ischemia and reperfusion 1 h group ( I/RⅢgroup);ischemia-reperfusion Ⅳgroup:120 min of ischemia and reperfusion 1 h group (I/RⅣgroup).Then all mice were killed and the brain glands were taken for immunohistochemistry staining and image analysis by computer .Results ①The result of immunohistochem-isty:the expression of Bcl-2 and Caspase-3 was pale brown in the brain neurons cells of mice of fasle operation group;the expression of Bcl-2 and Caspase-3 was brown yellow of I/R group;the expression of Bcl-2 and Caspase-3 was brown of I/R Ⅱgroup;compared with I/R Ⅱgroup, the expression of Bcl-2 was reduced significantly in I/R Ⅲgroup and I/RⅣgroup.The expression of Caspase-3 was dark brown in the neurons cells of mice of I/RⅢgroup;the expression of Caspase-3 was brown in I/RⅣgroup.②Comparison between the MOD of Bcl-2 in sham operation group and that of I/RⅡgroup was significant (P<0.05).The expression of Bcl-2 was significantly enhanced than that of I/R Ⅰand Ⅲgroup(P<0.05), the difference of between the MOD of Bcl-2 in I/RⅡgroup and that of I/RⅣgroup was significant(P<0.05).The MOD of Caspase-3 in I/RⅠgroup,I/RⅡgroup, I/RⅢgroup and I/RⅣgroup were compared with that of sham operation group , there were significant differences (P<0.05).the MOD of Caspase-3 in I/R Ⅱgroup and I/R Ⅲgroup were compared with that of I/RⅠgroup, there were significant differences (

关 键 词：缺血/再灌注损伤 BCL-2 CASPASE-3 脑 

分 类 号：R743[医药卫生—神经病学与精神病学]