MT01抑制Pg感染小鼠单核/巨噬细胞内NLRP3 mRNA的表达  

MT01 inhibits mRNA expression of NLRP3 after murine macrophage infection with Porphyromonas gingivalis

作　　者：郭恪[1] 申玉芹[1] 丁子清[1] 周岳[1] 于海蛟[1] 林崇韬[1] 

机构地区：[1]吉林大学口腔医学院牙周病科,吉林长春130021

出　　处：《中国老年学杂志》2014年第4期948-949,共2页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助课题(No.81371153);吉林省科技厅科研基金资助课题(No.201115105)

摘　　要：目的探讨MT01对牙龈卟啉单胞菌(Pg)感染小鼠单核/巨噬细胞NLRP3 mRNA表达的影响。方法选取小鼠单核/巨噬细胞RAW264.7为目的细胞,分别与Pg、MT01、Pg+MT01、磷酸盐缓冲液(PBS)共培养6 h,Real-time PCR检测NLRP3 mRNA的表达。结果与PBS组比较,NLRP3 mRNA的表达在MT01组最低(P<0.05),Pg组最高(P<0.01),Pg+MT01组高于PBS组,但低于Pg组(P<0.01)。结论 MT01可抑制Pg感染所导致的NLRP3 mRNA的表达。Objective To investigate the effect of MT01 on the NLRP3 mRNA expression in the murine macrophage after its infection with Porphyromonas gingivalis. Methods Real-time PCR was used to detect the mRNA expression of NLRP3 after cultivating the target cell RAW264. 7 with Pg,MT01,Pg + MT01,PBS respectively for 6 h. Results Compared with that of PBS group,the NLRP3 mRNA expression was significantly up-regulated by its culture with Porphyromonas gingivalis( P < 0. 01). After cultured with MT01,the expression value became the lowest among all the testing groups( P < 0. 05). However,after being cultured with Pg and MT01 together,the expression value was less up-regulated than culturing with Pg only. And it was more up-regulated than culturing with MT01 only( P < 0. 01). Conclusions MT01 could inhibit mRNA expression of NLRP3 after its infection with Pg.

关 键 词：NLRP3 MT01 牙龈卟啉单胞菌 RAW264.7 

分 类 号：R781.4[医药卫生—口腔医学]