乳腺癌细胞表面受体与CCL18结合促进肿瘤迁移的实验  

CCL18 Promotes Migration of Breast Cancer Cells via Binding to Its Functional Membrane Receptor

作　　者：陈静琦[1] 曾波航[1] 朱必胜[1] 侯开连[1] 

机构地区：[1]广州医学院第二附属医院肿瘤科,广州510260

出　　处：《肿瘤防治研究》2014年第2期134-137,共4页Cancer Research on Prevention and Treatment

基　　金：国家自然基金资助项目(81272900);广州市属高校科技计划项目资助课题(08A080)

摘　　要：目的探讨肿瘤相关巨噬细胞(TAM)来源的CCL18对乳腺癌迁移影响,为抑制乳腺癌迁移提供实验基础。方法用免疫荧光的方法检测CCL18与乳腺癌细胞表面受体结合及细胞骨架的变化;用迁移实验检测CCL18对乳腺癌迁移的作用。结果 CCL18与乳腺癌细胞表面受体结合,引起细胞骨架聚集;CCL18促进乳腺癌迁移。结论 CCL18通过其受体引起细胞骨架的聚集,促进乳腺癌细胞迁移。Objective To investigate the effects of CCL18 derived from tumor associated macrophages(TAM) on breast cancer migration, and to provide experimental basis to control the migration of breast cancer cells. Methods CCL18 binding to the membrane and causing the cytoskeleton change of breast cancer cells were detected by immunofluorescence. The migration assay was performed to detect CCL18 function in breast cancer migration. Results CCL18 binding to the membrane of breast cancer cells induced cytoskeleton gathering. Breast cancer cells migration was promoted by CCL18. Conclusion CCL18 binding to its receptor could promote breast cancer migration via causing cytoskeleton gathering.

关 键 词：乳腺癌细胞 CCL18 细胞骨架 迁移 

分 类 号：R737.9[医药卫生—肿瘤]