中人氟安局部植入治疗小鼠乳腺癌的实验  

5-Fluorouracil Delayed-release Implants in Local Treatment of Transplanted Breast Cancer of Balb/c Mice

作　　者：项华丽[1] 金梅[1] 唐新华[1] 潘铃娟[1] 

机构地区：[1]南昌大学第二附属医院乳腺外科,南昌330006

出　　处：《肿瘤防治研究》2014年第2期138-141,共4页Cancer Research on Prevention and Treatment

基　　金：江西省科技厅支撑计划项目(2009BSB11101)

摘　　要：目的研究氟尿嘧啶(5-Fu)缓释植入剂对乳腺癌移植瘤的治疗效果。方法将50只乳腺癌荷瘤小鼠随机分为5组,每组10只。A、B组于瘤旁植入5-Fu缓释植入剂,剂量分别为200和100 mg/kg;C组于瘤旁注射5-Fu注射液,剂量为200 mg/kg,D组于腹腔内注射5-Fu注射液200 mg/kg;E组不予任何治疗,为空白对照组。分别于给药后0、3、6、9和12 d观察荷瘤小鼠生存情况、体质量变化及肿瘤体积,12天后处死所有小鼠,称瘤重,计算抑瘤率,绘制肿瘤生长曲线,完整剥离瘤块经福尔马林浸泡固定后制作石蜡切片进行免疫组织化学观察肿瘤细胞HER2表达情况。结果 (1)A、B组肿瘤生长曲线平缓,12天后A、B、C及D组抑瘤率分别为50.43%,41.64%,27.57%和22.87%;(2)A、B组肿瘤体积与C、D组比较,差异有统计学意义(P<0.05);(3)氟尿嘧啶(5-Fu)缓释植入剂瘤周植入组ErbB2表达率明显低于对照组,差异有统计学意义(P<0.05)。结论 5-Fu缓释植入剂于瘤旁植入能安全有效地抑制乳腺癌的生长。Objective To compare the effi cacies of 5-fl uorouracil(5-Fu) delayed-release implants in local treatment of transplanted breast cancer of Balb/c mice. Methods Fifty Balb/c mice with transplanted breast cancer were randomly divided into 5 groups, 10 in each group. Group A and B were treated with peritumoral implantation of 5-Fu implants, 200 and 100 mg/kg, respectively. Group C was treated with peritumoral injection of 5-Fu solution,200 mg/kg. Group D was treated with intra-abdominal injection of 5-Fu solution, 200 mg/kg. Group E did not receive any treatment. The survival, bodyweight and tumor volume were observed after treatment for 0,3,6,9 and 12 d.After 12 days, all mice were killed, tumors were weighed, tumor inhibition rates were calculated, growth curves were plotted and HER2 expression were detected by immunohistochemistry. Results The growth curves were mild in group A and B. After 12 days, inhibition rates of group A,B,C and D were 50.43%, 41.64%, 27.57% and 22.87%, respectively.There were statistical differences in tumor volume between group A,B and other groups(P<0.05), while there was no statistical difference among group C,D or E. The HER2 expressions in group A,B and C were signifi cantly lower than that in group D(P<0.05).Conclusion Delayed-release 5-fluoroumcil implants can effectively inhibit the growth of breast cancer.

关 键 词：乳腺癌 氟尿嘧啶缓释剂 HER2 BALB/C小鼠 

分 类 号：R737.9[医药卫生—肿瘤]