大鼠热结肠道型气分证模型的构建及肠道结构、IEL和GC数量的变化研究  

Study on Construction of Heat Accumulation of Intestinal Type of Qi-fen Syndrome and Changes of Intestinal Structure and Number of IEL,GC in Rat

作　　者：王自力[1,2] 贺庭祯 张翥[1] 王陈超 王明周[1] 涂君平 谢尔玛 张家骅[1] 别应堂 

机构地区：[1]西南大学动物科技学院,重庆400715 [2]重庆市光大(集团)有限公司,重庆400020

出　　处：《畜牧兽医学报》2014年第4期672-678,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然基金青年基金项目(31001082);教育部博士点基金(20100182120009);重庆市博士后科研项目特别资助项目(XM20120050)

摘　　要：为了建立大鼠热结肠道型气分证模型,通过给大鼠灌服热性中药、自身粪便悬液及腹腔注射灭活大肠杆菌菌液,分别观察和检测大鼠临床症状、采食量、饮水量、体重、体温变化,并对大鼠肠道组织结构、IEL和GC数量变化进行研究。结果表明,灌服温热性中药、自身粪便及注射灭活大肠杆菌液后5h时大鼠临床症状、采食量、饮水量及体温等均符合中兽医热结肠道型气分证特征,肠道组织结构变化以注射灭活大肠杆菌后5h病理损伤最为明显,且十二指肠、空肠、回肠的IEL和杯状细胞数量均出现显著下降。即确定灌服12d热性中药及2d大鼠自身粪便后,腹腔注射灭活大肠杆菌后5h可成功制作出热结肠道型气分证大鼠模型。In order to construct the model of heat accumulation of intestinal type of Qi-fen Syndrome,the rats were feed with the hot medicine and the rat wastes,then were injected with the inactive E.coli,and the clinic symptoms such as the in taking,drinking,body weight and body temperature were determined,even the intestinal organizational structure,and the number of intestinal IEL and GC in rat were studied.According to the clinic symptoms,in taking,drinking,body temperature of the rats,the results showed that that the methods of feeding with the hot medicine,wastes and injecting the inactive E.coli to the rats could make the model of heat accumulation of intestinal type of Qi-fen Syndrome in successfully when the rats were injected with the inactive E.coli 5hours later.The changes of intestinal structure in rat injected with the inactive E. coli 5hours later were significantly,and the numbers of the IEL and GC in the duodenum,jejunum and ileum decreased in significantly.Therefore,the model of heat accumulation of intestinal type of Qi-fen Syndrome in rat can be made in successfully after the rats were injected with the inactive E.coli 5hours later.

关 键 词：大鼠 热结肠道型气分证 动物模型 肠道组织结构 IEL GC 

分 类 号：S858.91[农业科学—临床兽医学]