The unmediated choline sensor based on layered double hydroxides in hydrogen peroxide detection mode  

作　　者：DING ShouNian SHAN Dan YAO WenJuan XUE HuaiGuo SUN YueMing COSNIER Serge 

机构地区：[1]School of Chemistry&Chemical Engineering,Southeast University,Nanjing 211189,China [2]Key Laboratory of Environmental Materials&Environmental Engineering of Jiangsu Province,Yangzhou 225002,China [3]School of Chemistry&Chemical Engineering,Yangzhou University,Yangzhou 225002,China [4]Départment de Chimie Moléculaire UMR-5250,ICMG FR-2607,CNRS UniversitéJoseph Fouier,BP-53,Grenoble 38041,France

出　　处：《Science China Chemistry》2009年第12期2281-2286,共6页中国科学（化学英文版）

基　　金：Supported by the National Natural Science Foundation of China (Grant Nos. 20505014 & 20773108);Jiangsu Science and Technology Development Foundation (Grant No. BE2007014);Jiangsu Natural Science Foundation (Grant No. BK2007563)

摘　　要：In this work,we have developed a novel choline biosensor on the basis of immobilization of choline oxidase (ChOx) by the attractive materials layered double hydroxides (LDHs). Amperometric detection of choline was evaluated by holding the modified electrode at 0.5 V (vs. SCE). Due to the special properties of LDHs ([Zn3-Al-Cl]),such as chemical inertness,high porosity,and swelling property,the [Zn3-Al-Cl]/ChOx modified electrode exhibited an enhanced analytical performance. The biosensor provided a linear response to choline over a concentration range from 3.7 × 10-6 to 6.3 × 10-4 M with a low detection limit of 3 × 10-7 M based on S/N=3. The apparent Michaelis-Menten constant was calculated to be 1.38 mM. In addition,the interaction between ChOx and LDHs has also been investigated using FT-IR spectroscopy.In this work, we have developed a novel choline biosensor on the basis of immobilization of choline oxidase (ChOx) by the attractive materials layered double hydroxides (LDHs). Amperometric detection of choline was evaluated by holding the modified electrode at 0.5 V (vs. SCE). Due to the special properties of LDHs ([Zn3-Al-Cl]), such as chemical inertness, high porosity, and swelling property, the [Zn3-Al-Cl]/ChOx modified electrode exhibited an enhanced analytical performance. The biosensor provided a linear response to choline over a concentration range from 3.7 × 10?6 to 6.3 × 10?4 M with a low detection limit of 3 × 10?7 M based on S/N=3. The apparent Michaelis-Menten constant was calculated to be 1.38 mM. In addition, the interaction between ChOx and LDHs has also been investigated using FT-IR spectroscopy.

关 键 词：layered double HYDROXIDES clay CHOLINE OXIDASE CHOLINE BIOSENSOR 

分 类 号：TP212.2[自动化与计算机技术—检测技术与自动化装置]