The three-dimensional structure of Infectious flacherie virus capsid determined by cryo-electron microscopy  

作　　者：XIE Li1,4, ZHANG QinFen2·, LU XingMeng3, DAI XinHong2, LI KunPeng2, HONG Jian1,4· & ZHOU XuePing11 Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China 2 State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China 3 College of Animal Sciences, Zhejiang University, Hangzhou 310029, China 4 Center of Analysis and Measurement, Zhejiang University, Hangzhou 310029, China 

出　　处：《Science China(Life Sciences)》2009年第12期1186-1191,共6页中国科学（生命科学英文版）

基　　金：Supported by the National Natural Science Foundation of China (Grant No. 30370305);the National Basic Research and Development Program of China (Grant No. 2005CB121003)

摘　　要：Cryo-electron microscopy and image reconstruction were used to determine the three-dimensional structure of Infectious flacherie virus (IFV). 5047 particles were selected for the final reconstruction. The FSC curve showed that the resolution of this capsid structure was 18 ·. The structure is a psuedo T=3 (P=3) icosahedral capsid with a diameter of 302.4 · and a single shell thickness of 15 ·. The density map showed that IFV has a smooth surface without any prominent protrude or depression. Comparison of the IFV structure with those of the insect picorna-like virus-Cricket paralysis virus (CrPV)and human picornavirus-Human rhinovirus 14 (HRV 14) revealed that the IFV structure resembles the CrPV structure. The "Rossmann canyon" is absent in both IFV and CrPV particles. The polypeptide topology of IFV VP2, IFV VP3 was predicted and the subunit location at the capsid surface was further analyzed.Cryo-electron microscopy and image reconstruction were used to determine the three-dimensional structure of Infectious flacherie virus (IFV). 5047 particles were selected for the final reconstruction. The FSC curve showed that the resolution of this capsid structure was 18 ·. The structure is a psuedo T=3 (P=3) icosahedral capsid with a diameter of 302.4 · and a single shell thickness of 15 ·. The density map showed that IFV has a smooth surface without any prominent protrude or depression. Comparison of the IFV structure with those of the insect picorna-like virus-Cricket paralysis virus (CrPV)and human picornavirus-Human rhinovirus 14 (HRV 14) revealed that the IFV structure resembles the CrPV structure. The 'Rossmann canyon' is absent in both IFV and CrPV particles. The polypeptide topology of IFV VP2, IFV VP3 was predicted and the subunit location at the capsid surface was further analyzed.

关 键 词：INFECTIOUS FLACHERIE VIRUS cryo-electron microscopy three-dimensional STRUCTURE POLYPEPTIDE folding 

分 类 号：Q939.4[生物学—微生物学]