牡蛎肉提取物对类固醇性骨质疏松大鼠骨代谢的影响  

Effects of Oyster Extract on Bone Metabolism in Rats with Glucocorticoid-induced Osteoporosis

作　　者：苏开鑫[1] 谢华[1] 王宏芬[1] 陈海芳[1] 

机构地区：[1]广东医学院解剖学教研室,广东湛江524023

出　　处：《中国病毒病杂志》2009年第2期97-99,共3页Chinese Journal of Viral Diseases

摘　　要：目的探讨牡蛎肉提取物对类固醇性骨质疏松大鼠骨代谢的影响。方法40只3月龄SD大鼠,随机分成年龄对照组(A组)、泼尼松组(B组)、低剂量牡蛎组(C组)、高剂量牡蛎组(D组)。A组灌生理盐水4mg·kg-1.d-1,B组予4mg·kg-1.d-1泼尼松灌胃,C组灌胃给予牡蛎肉提取物5g·kg-1.d-1+醋酸泼尼松4mg·kg-1.d-1,D组给予牡蛎肉提取物10g·kg-1.d-1+醋酸泼尼松4mg·kg-1.d-1,共90d。实验结束后,处死全部大鼠,取尺骨和血清做生化指标测定。结果泼尼松可致大鼠尺骨羟脯氨酸的含量减少,骨钙、骨磷、骨锌、骨铁的含量显著降低;血钙显著升高,碱性磷酸酶含量降低。牡蛎提取物可提高大鼠骨钙、骨磷、骨锌、骨铁含量;使血钙降低恢复正常,碱性磷酸酶浓度升高,各项指标均接近正常对照组水平,且高剂量组比低剂量组效果更明显。结论牡蛎肉提取物可以有效防治泼尼松引起的骨代谢紊乱。Objective To study the effects of oyster extract on bone metabolism in rats with glucocorticoid-induced osteoporosis. Methods Forty three-month-old male Sprague-dawley rats were divided into four groups randomly:normal group(group A),prednisone group(group B),low oyster group(group C),and high oyster group(group D).Group A was given 4 mg·kg-1·d-1 of normal saline;Group B was given 4 mg·kg-1·d-1 of prednisone;Group C was given 5 g·kg-1·d-1 of oyster+4 mg·kg-1·d-1 of prednisone;Group D was given 10 g·kg-1·d-1of oyster+4 mg·kg-1·d-1 of prednisone.Ninety days later,all rats were killed and concentrations of ulna hydroxyproline(Hyp),calcium,phosphorus,zinc,and ferrum were measured as well as levels of serum calcium,phosphorus,alkaline phosphatase(ALP),alanine aminotransferase. Results Prednisone significantly decreased the concentrations of ulna calcium,phosphorus,zinc,ferrum and serum ALP,and enhanced level of serum calcium in rats(P all<0.05).Although a slight decrease in the concentration of ulna Hyp induced by prednisone was found,the difference was not statistically significant.Oyster extract significantly enhanced concentrations of ulna calcium,phosphorus,zinc,ferrum and serum ALP,and recovered lowered serum calcium.The effects of oyster extract were more significant in high-dose group than those in low-dose group. Conclusion Oyster extract has significant prophylactic and therapeutic effects on prednisone-induced osteoporosis.

关 键 词：牡蛎肉 糖皮质激素 骨代谢 骨质疏松 

分 类 号：R580[医药卫生—内分泌]