Phylogenetic status of Pneumocystis from corticosteroid-treated gerbils  

作　　者：RODNEY D Adam 

机构地区：[1]Department of Medicine and Immunobiology, University of Arizona College of Medicine

出　　处：《Science China(Life Sciences)》2010年第10期1239-1246,共8页中国科学（生命科学英文版）

基　　金：supported by the Natural Science Foundation of Beijing (Grant No. 7052009)

摘　　要：Pneumocystis spp. infect the lungs of multiple mammalian species and cause disease in immunosuppressed individuals. The Pneumocystis isolates that have been studied to date fall into two major clades, those from primates and those from rodents. Within each of these clades, different species have been described on the basis of host specificity and differences in sequence and morphology. Here, we demonstrate that dexamethasone immunosuppression consistently results in histologically apparent lung infection in gerbils (28/35 animals). Sequence analysis of the 18S, 5.8S and internal transcribed spacer regions of the rDNA and a portion of the mitochondrial large subunit rDNA demonstrated that this gerbil Pneumocystis is grouped with other rodent Pneumocystis spp., but is distinct from them. Our results suggest that gerbil Pneumocystis differs sufficiently from Pneumocystis species found in other rodents to be considered a separate species.Pneumocystis spp. infect the lungs of multiple mammalian species and cause disease in immunosuppressed individuals. The Pneumocystis isolates that have been studied to date fall into two major clades, those from primates and those from rodents. Within each of these clades, different species have been described on the basis of host specificity and differences in sequence and morphology. Here, we demonstrate that dexamethasone immunosuppression consistently results in histologically apparent lung infection in gerbils (28/35 animals). Sequence analysis of the 18S, 5.8S and internal transcribed spacer regions of the rDNA and a portion of the mitochondrial large subunit rDNA demonstrated that this gerbil Pneumocystis is grouped with other rodent Pneumocystis spp., but is distinct from them. Our results suggest that gerbil Pneumocystis differs sufficiently from Pneumocystis species found in other rodents to be considered a separate species.

关 键 词：5.8S RRNA GENE GERBIL internal transcribed spacers (ITS) mitochondrial large subunit (mtLS) RRNA GENE 18S RRNA GENE PNEUMOCYSTIS 

分 类 号：R563.1[医药卫生—呼吸系统]