Preparation of micro-patterned surfaces of Si-N-O films and their influence on adhesion behavior of endothelial cells  

作　　者：ZHENG Nan,YANG Ping,WANG QiYi,YANG ZhongHai & HUANG Nan Key Laboratory of Advanced Technology for Materials of Education Ministry,Key Laboratory of Artificial Organ Surface Engineering of Sichuan,School of Materials Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China 

出　　处：《Science China(Technological Sciences)》2010年第1期257-263,共7页中国科学（技术科学英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant Nos.30570502,30870629)

摘　　要：Microgrooves were prepared on Si(100) surface by photolithography and wet etching.Subsequently,Si-N-O films were deposited on the microgrooves by unbalanced magnetron sputtering(UBMS) and micro-patterned surfaces of Si-N-O films were obtained.The size of the micropatterns was measured by surface profilometer.The chemical composition of Si-N-O films were characterized by X-ray photoelectron spectrometry(XPS) and the wettability of the micropatterned surfaces was evaluated by contact angle measurement.The behavior of endothelial cells adhered on the micro-patterned surfaces,including cells adhesion,proliferation and orientation,was evaluated by cell culture test and Alamar blue assay.The results showed that the amount and activity of endothelial cells on micro-patterned samples were higher than those on flat samples.After one day's incubation,the cells were well guided along the microgrooves.Three days later,most of the cells grew across the grooves and contacted each other.Their activity was also much higher than that on flat samples.It was suggested that the adhesion and proliferation of endothelial cells could be effectively enhanced by micropattern method.Microgrooves were prepared on Si(100) surface by photolithography and wet etching.Subsequently,Si-N-O films were deposited on the microgrooves by unbalanced magnetron sputtering(UBMS) and micro-patterned surfaces of Si-N-O films were obtained.The size of the micropatterns was measured by surface profilometer.The chemical composition of Si-N-O films were characterized by X-ray photoelectron spectrometry(XPS) and the wettability of the micropatterned surfaces was evaluated by contact angle measurement.The behavior of endothelial cells adhered on the micro-patterned surfaces,including cells adhesion,proliferation and orientation,was evaluated by cell culture test and Alamar blue assay.The results showed that the amount and activity of endothelial cells on micro-patterned samples were higher than those on flat samples.After one day’s incubation,the cells were well guided along the microgrooves.Three days later,most of the cells grew across the grooves and contacted each other.Their activity was also much higher than that on flat samples.It was suggested that the adhesion and proliferation of endothelial cells could be effectively enhanced by micropattern method.

关 键 词：MICROPATTERNS Si-N-O film ENDOTHELIAL cell unbalanced MAGNETRON SPUTTERING 

分 类 号：TB383.2[一般工业技术—材料科学与工程]