Simultaneous determination of five anti-epilepsy drugs in human plasma using liquid chromatography-mass spectrometry  

作　　者：STEFANIE WeiBig 

机构地区：[1]Institute of Pharmaceutical Analysis,College of Pharmacy,Wuhan University

出　　处：《Science China Chemistry》2010年第11期2373-2378,共6页中国科学（化学英文版）

基　　金：supported by the National Science & Technology Major Project (2009ZX09301-14);the National Natural Science Foundation of China (90817103,20775055 & 30973672);the Startup Funding for ZC’s Luojia Chair Professorship of Wuhan University (306276216 & 306271159);the Fundamental Research Funds for the Central Universities

摘　　要：A new liquid chromatography-mass spectrometry method for the determination of carbamazepine,clonazepam,alprazolam,estazolam and phenytoin in human plasma has been developed by using diazepam as an internal standard.Chromatographic separation was performed on a Zorbax SB-C18 column(30 mm × 2.1 mm,3.5 ?m) with a mobile phase consisting of methanol and aqueous 25 mM ammonium acetate using gradient elution.A diethyl ether extraction method was used for the extraction of five anti-epilepsy drugs.The final extract was injected for analysis by LC-MS/MS.The method was validated within the concentration range of 50-5000 ng mL-1 for five anti-epilepsy drugs.The precision of the assay(RSD%) was less than 10% at all concentration levels within the tested range.The method recoveries for all samples were more than 90%.The results indicate that the method is specific,sensitive and accurate,and suitable to study the pharmacokinetics,to adjust the dosage for individual administration,and to monitor the drug-concentration and drug abuse of the five anti-epilepsy drugs.A new liquid chromatography-mass spectrometry method for the determination of carbamazepine,clonazepam,alprazolam,estazolam and phenytoin in human plasma has been developed by using diazepam as an internal standard.Chromatographic separation was performed on a Zorbax SB-C18 column(30 mm × 2.1 mm,3.5 ?m) with a mobile phase consisting of methanol and aqueous 25 mM ammonium acetate using gradient elution.A diethyl ether extraction method was used for the extraction of five anti-epilepsy drugs.The final extract was injected for analysis by LC-MS/MS.The method was validated within the concentration range of 50-5000 ng mL-1 for five anti-epilepsy drugs.The precision of the assay(RSD%) was less than 10% at all concentration levels within the tested range.The method recoveries for all samples were more than 90%.The results indicate that the method is specific,sensitive and accurate,and suitable to study the pharmacokinetics,to adjust the dosage for individual administration,and to monitor the drug-concentration and drug abuse of the five anti-epilepsy drugs.

关 键 词：liquid chromatography-mass SPECTROMETRY anti-epilepsy DRUGS plasma CONCENTRATION 

分 类 号：R965[医药卫生—药理学]