Crosslinking of viral nanoparticles with “clickable” fluorescent crosslinkers at the interface  

作　　者：KAUR Gagandeep BARNHILL Hannah 

机构地区：[1]Department of Chemistry and Biochemistry and Nanocenter,University of South Carolina

出　　处：《Science China Chemistry》2010年第6期1287-1293,1459,共8页中国科学（化学英文版）

基　　金：We are grateful for the support from the US NSF CAREER Award (CHE-0748690);US DoD–ARO (W911NF-09-1-0236), US DoE;the W. M. Keck Foundation;the Camille Dreyfus Teacher-Scholarship;the Alfred P. Sloan Foundation;the National Natural Science Foundation of China (50673025);the National Basic Research 973 Program (2006CB806200)

摘　　要：Cu (I) catalyzed alkyne-azide cycloaddition (CuAAC) reaction,a typical "click" reaction,is one of the modular synthetic approaches which has been broadly used in various organic syntheses,medicinal chemistry,materials development and bioconjugation applications.We have for the first time synthesized two dialkyne derivatized fluorescent crosslinkers which could be applied to crosslink two biomolecules using CuAAC reaction.Turnip yellow mosaic virus,a plant virus with unique structural and chemical properties,was used as a prototypical scaffold to form a 2D single layer at the interface of two immiscible liquids and crosslinked with these two linkers by the CuAAC reaction.Upon crosslinking,the fluorescence of both linkers diminished,likely due to the distortion of the polymethylene backbone,which therefore could be used to indicate the completion of the reaction.Cu (I) catalyzed alkyne-azide cycloaddition (CuAAC) reaction,a typical "click" reaction,is one of the modular synthetic approaches which has been broadly used in various organic syntheses,medicinal chemistry,materials development and bioconjugation applications.We have for the first time synthesized two dialkyne derivatized fluorescent crosslinkers which could be applied to crosslink two biomolecules using CuAAC reaction.Turnip yellow mosaic virus,a plant virus with unique structural and chemical properties,was used as a prototypical scaffold to form a 2D single layer at the interface of two immiscible liquids and crosslinked with these two linkers by the CuAAC reaction.Upon crosslinking,the fluorescence of both linkers diminished,likely due to the distortion of the polymethylene backbone,which therefore could be used to indicate the completion of the reaction.

关 键 词：PICKERING emulsion CROSSLINKING NANOPARTICLES CUAAC reaction CLICK chemistry interfacial assembly 

分 类 号：TB383.1[一般工业技术—材料科学与工程]