Cpb1基因在乳腺癌中差异表达的初步研究  

Preliminary study of differential expression of Cpb1 genes in breast cancer

作　　者：金霆[1] 费政芳[1] 

机构地区：[1]福建省福州市第一医院,福建福州350009

出　　处：《分子诊断与治疗杂志》2009年第1期10-13,共4页Journal of Molecular Diagnostics and Therapy

摘　　要：目的本研究利用生物信息学,从GenBank的乳腺癌表达文库中筛选了未见于国内外报道的高表达基因进行初步研究,以发现与乳腺癌相关的新基因。方法利用RT-PCR法对福州市第一医院和福建省人民医院乳腺外科2006～2007年切除手术中收集到的25例乳腺癌组织、11例癌旁正常组织和6例良性肿物组织进行了测定。重点研究Cpb1基因在乳腺癌组织、癌旁正常组织、良性肿物组织的表达差异。结果通过Fisher’s精确检验,Cpb1在小部分乳腺癌组织中具有特异性表达。结论研究表明Cpb1基因在部分乳腺癌患者中其表达差异显著,提示有望成为新的乳腺癌分子标志物。Objective To study the differential expression of Cpb1 genes in breast cancer. Methods RT-PCR were used to examine the expression level of Cpb1 genes in samples collected from 25 patients with breast cancer. The differential expression of Cpb1 gene among caner tissue, adjacent non-neoplastic tissue and benign neoplasm were compared. Results Cpb1 gene was differentially over-expression in a rather small portion of the samples(20%). Conclusion The novel gene markers of breast cance in early diagnosis may be significant.

关 键 词：乳腺肿瘤 差异表达 Cpb1 

分 类 号：R737.9[医药卫生—肿瘤]