新型聚乙烯亚胺衍生物在Hep G2细胞中转染和毒性的研究  

Transfection and Cytotoxicity Studies of Novel Polyethyleneimine Derivative on Hep G2

作　　者：何沐[1] 何倩倩[1] 刘成容[2] 吕霜洁 苏靖[1] 

机构地区：[1]上海交通大学药学院,上海200240 [2]吉林大学生命科学学院,吉林长春130012

出　　处：《现代生物医学进展》2009年第24期4613-4614,共2页Progress in Modern Biomedicine

基　　金：国家自然科学基金(30873180)

摘　　要：目的:研究以1,4-丁二醇二氯甲酸酯为连接剂的聚乙烯亚胺(Polyethyleneimine,PEI)衍生物PEI-Bu对肝癌细胞的转染活性和细胞毒性的影响。方法:以荧光素酶质粒作为报告基因,研究高分子和DNA的复合物在HepG2细胞中的转染活性,研究高分子对Hep G2细胞的毒性。结果:Hep G2细胞转染结果显示我们构建的聚乙烯亚胺衍生物PEI-Bu有比阳性对照PEI25 kDa和Lipofectamine 2000更高的基因表达;细胞毒性结果显示PEI-Bu随着浓度的增加,其毒性显著低于PEI25 kDa。结论:Hep G2细胞实验数据显示PEI-Bu是一种高效、低毒,在肝脏基因治疗领域有相当前景的非病毒载体。Objective: To investigate the transfection efficiency and cell toxicity of Polyethyleneimine (PEI) derivative linking with 1, 4-Dibutanediol Chloroformate. Methods: Luciferase plasmid was used as the reporter gene to investigate transfection efficiency in Hep G2 cells, cytotoxicity experiments were carried out in this cell line either. Results: Transfection results indicated that the gene carrier (PEI-Bu) performed better than the positive control of PEI 25kDa and Lipofectamine 2000. Cytotoxicity result suggested that PEI-Bu was less cytotoxic than PEI 25kDa with the increase of polymer concentration. Conclusion: Transfection and cytotoxicity data of Hep G2 indicated that PEI-Bu could be a highly efficient and less toxic gene carrier, which was promising in liver gene therapy.

关 键 词：1 4-丁二醇二氯甲酸酯 聚乙烯亚胺 HEP G2 转染 细胞毒性 

分 类 号：R735.7[医药卫生—肿瘤]