TCR基因重排在T淋巴细胞浸润亲表皮疾病中的应用  

TCR gene rearrangements in T-lymphocyte infiltration in skin diseases of the pro effective way

作　　者：孙亮亮[1] 普雄明[2] 

机构地区：[1]石河子大学医学院,新疆石河子832002 [2]新疆维吾尔自治区人民医院皮肤性病科,乌鲁木齐830001

出　　处：《现代生物医学进展》2009年第24期4754-4755,共2页Progress in Modern Biomedicine

摘　　要：目的:探讨具有亲表皮现象的疾病T细胞受体γ链基因重排的情况。方法:用免疫组化标记筛选17例T细胞淋巴瘤、30例可疑为T细胞淋巴瘤和10例副银屑病,采用聚合酶链式反应扩增方法检测T细胞受体γ链基因重排。结果:17例T细胞淋巴瘤中12例出现T细胞受体γ链基因重排,30例可疑为T细胞淋巴瘤和10例副银屑病均无T细胞受体γ链基因重排。结论:T细胞受体γ链基因重排检测是区分T淋巴细胞浸润的亲表皮疾病鉴别诊断的有效方法,且对T细胞淋巴瘤的确诊有重要参考价值与显著意义。Objective:To explore the rearrangement of T cell receptor geneγ chain ( TCRγ) in the phenomenon of skin diseases with the pro effective way. Methods:Specimens f rom 17 cases of T cell lymphoma ,30 cases that were difficult in diagnosis(malignant versus reactive T2cell infilt rate) , and 10 cases of lymphaden reaction hyperplasia (RH) . All above specimens were studied by immunohistochemical (IHC) methods and their TCRγgene rearrangement s were detected by polymerase chain reaction (PCR) technique. Results:17 cases of T-cell lymphoma, 12 patients had T-cell receptor γ chain gene rearrangement, 30 cases suspicious for T-cell lymphoma and 10 cases of parapsoriasis no T-cell receptor γ chain gene rearrangement. Conclusion:T-cell receptor γ chain gene rearrangement detection is to distinguish between T-lymphocyte infiltration in skin diseases, differential diagnosis of pro-effective way, and the pairs of T-cell lymphoma diagnosis was significant

关 键 词：亲表皮现象 T细胞淋巴瘤 T细胞受体γ链 聚合酶链式反应 基因重排 

分 类 号：R733.1[医药卫生—肿瘤]